In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH

Monoxenic cultures of arbuscular mycorrhizal fungi (AMF) in transformed roots have been used to better understand the symbiosis with these fungi, but few species have been successfully established in vitro. The object was to establish monoxenic cultures of Gigaspora decipiens and Glomus clarum and t...

Descripción completa

Detalles Bibliográficos
Autores: Kasuya, Catarina Megumi, Costa, Francilina Araújo, Haddad, Lydice Sant'Anna Meira, Oton, Wagner Campos, Costa, Maurício Dutra, Borges, Arnaldo Chaer
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Universidade Federal de Viçosa (UFV)
Repositorio:LOCUS Repositório Institucional da UFV
Idioma:inglés
OAI Identifier:oai:locus.ufv.br:123456789/25189
Acceso en línea:http://dx.doi.org/10.4025/actasciagron.v35i3.16581
http://www.locus.ufv.br/handle/123456789/25189
Access Level:acceso abierto
Palabra clave:Arbuscular mycorrhizal fungi
Monoxenic culture
In vitro culture
Monoxenic inoculum
Transformed roots
Fungos micorrízicos arbusculares
Cultura monoxênica
Cultura in vitro
Inoculum monoxênico
Raízes transformadas
id BR_7d0987e4abdd1c1b8582d0cc69ae08fe
oai_identifier_str oai:locus.ufv.br:123456789/25189
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pHArbuscular mycorrhizal fungiMonoxenic cultureIn vitro cultureMonoxenic inoculumTransformed rootsFungos micorrízicos arbuscularesCultura monoxênicaCultura in vitroInoculum monoxênicoRaízes transformadasMonoxenic cultures of arbuscular mycorrhizal fungi (AMF) in transformed roots have been used to better understand the symbiosis with these fungi, but few species have been successfully established in vitro. The object was to establish monoxenic cultures of Gigaspora decipiens and Glomus clarum and to verify the effects of temperature and pH on spore formation. Cultures were established from superficially disinfested spores that were germinated on an agar and water. After germination, the spores were transferred to Petri dishes containing transformed carrot roots. After 4-6 days formed newly formed spores and structures typical. The effects of temperature (22, 25, 28 or 32ºC) and pH (4.0, 4.5, 5.5 or 6.5) on the production of spores were assessed over three months, resulting in sigmoidal growth curves. The spore increased from 22ºC, reaching higher values 28oC and 32oC there was a reduction in the production. The highest spore production of G. decipiens occurred in a pH of 6.5, whereas for G. clarum was pH of 4.0. The cultivation of other species is still necessary to elucidate particular aspects of the symbiosis that so far are unclear, including the effects of environmental factors on the production of spores of different AMF species.Culturas monoxênicas de fungos micorrízicos arbusculares (FMAs) em raízes transformadas têm sido utilizadas para melhor compreensão desses fungos, porém, poucas espécies foram estabelecidas in vitro com sucesso. O objetivo do trabalho foi estabelecer culturas monoxênicas de Gigaspora decipiens e Glomus clarum e verificar o efeito da temperatura e pH na produção de esporos. As culturas foram obtidas de esporos desinfestados e germinados em Agar-água. Após a germinação foram transferidos para placas de Petri contendo raízes de cenoura transformadas, em meio mínimo. Após 4 a 6 dias formou-se novos esporos e estruturas típicas. O efeito da temperatura (22, 25, 28 e 32ºC) e pH (4,0; 4,5; 5,5 e 6,5) na produção de esporos foram analisados por 3 meses, obtendo-se curvas sigmoidais. Os esporos aumentaram a partir de 22ºC, atingindo valores maiores em 28ºC e em 32ºC reduziu-se a produção. A maior produção de esporos de G. decipiens ocorreu em pH 6,5, enquanto G. clarum em pH 4,0. O cultivo de outras espécies faz-se necessário visando o conhecimento de aspectos particulares da simbiose até hoje não elucidados, incluindo-se os efeitos dos fatores ambientais sobre a produção dos esporos das diversas espécies de FMAs.Acta Scientiarum. Agronomy2019-05-15T13:00:45Z2019-05-15T13:00:45Z2013-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf18078621http://dx.doi.org/10.4025/actasciagron.v35i3.16581http://www.locus.ufv.br/handle/123456789/25189engv. 35, n. 03, p. 315- 323, jul./ sept. 2013info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVKasuya, Catarina MegumiCosta, Francilina AraújoHaddad, Lydice Sant'Anna MeiraOton, Wagner CamposCosta, Maurício DutraBorges, Arnaldo Chaer2024-07-12T07:32:46Zoai:locus.ufv.br:123456789/25189Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:32:46LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
title In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
spellingShingle In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
Kasuya, Catarina Megumi
Arbuscular mycorrhizal fungi
Monoxenic culture
In vitro culture
Monoxenic inoculum
Transformed roots
Fungos micorrízicos arbusculares
Cultura monoxênica
Cultura in vitro
Inoculum monoxênico
Raízes transformadas
title_short In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
title_full In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
title_fullStr In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
title_full_unstemmed In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
title_sort In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH
dc.creator.none.fl_str_mv Kasuya, Catarina Megumi
Costa, Francilina Araújo
Haddad, Lydice Sant'Anna Meira
Oton, Wagner Campos
Costa, Maurício Dutra
Borges, Arnaldo Chaer
author Kasuya, Catarina Megumi
author_facet Kasuya, Catarina Megumi
Costa, Francilina Araújo
Haddad, Lydice Sant'Anna Meira
Oton, Wagner Campos
Costa, Maurício Dutra
Borges, Arnaldo Chaer
author_role author
author2 Costa, Francilina Araújo
Haddad, Lydice Sant'Anna Meira
Oton, Wagner Campos
Costa, Maurício Dutra
Borges, Arnaldo Chaer
author2_role author
author
author
author
author
dc.subject.por.fl_str_mv Arbuscular mycorrhizal fungi
Monoxenic culture
In vitro culture
Monoxenic inoculum
Transformed roots
Fungos micorrízicos arbusculares
Cultura monoxênica
Cultura in vitro
Inoculum monoxênico
Raízes transformadas
topic Arbuscular mycorrhizal fungi
Monoxenic culture
In vitro culture
Monoxenic inoculum
Transformed roots
Fungos micorrízicos arbusculares
Cultura monoxênica
Cultura in vitro
Inoculum monoxênico
Raízes transformadas
description Monoxenic cultures of arbuscular mycorrhizal fungi (AMF) in transformed roots have been used to better understand the symbiosis with these fungi, but few species have been successfully established in vitro. The object was to establish monoxenic cultures of Gigaspora decipiens and Glomus clarum and to verify the effects of temperature and pH on spore formation. Cultures were established from superficially disinfested spores that were germinated on an agar and water. After germination, the spores were transferred to Petri dishes containing transformed carrot roots. After 4-6 days formed newly formed spores and structures typical. The effects of temperature (22, 25, 28 or 32ºC) and pH (4.0, 4.5, 5.5 or 6.5) on the production of spores were assessed over three months, resulting in sigmoidal growth curves. The spore increased from 22ºC, reaching higher values 28oC and 32oC there was a reduction in the production. The highest spore production of G. decipiens occurred in a pH of 6.5, whereas for G. clarum was pH of 4.0. The cultivation of other species is still necessary to elucidate particular aspects of the symbiosis that so far are unclear, including the effects of environmental factors on the production of spores of different AMF species.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
2019-05-15T13:00:45Z
2019-05-15T13:00:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 18078621
http://dx.doi.org/10.4025/actasciagron.v35i3.16581
http://www.locus.ufv.br/handle/123456789/25189
identifier_str_mv 18078621
url http://dx.doi.org/10.4025/actasciagron.v35i3.16581
http://www.locus.ufv.br/handle/123456789/25189
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 35, n. 03, p. 315- 323, jul./ sept. 2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Acta Scientiarum. Agronomy
publisher.none.fl_str_mv Acta Scientiarum. Agronomy
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1853667140592205824
score 15.198674

Similares en LA Referencia