Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation

Silva, Alexandre Rodrigues; Cardoso, Rita de Cássia Soares; Silva, Lúcia Daniel Machado da

Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation

The present study compared powder coconut water (ACP®) and Tris extenders on canine semen cryopreservation by classic evaluation and thermoresistance test. Five stud dogs were submitted to two semen collections by digital manipulation. Sperm fractions were analyzed regarding to its color, volume, sp...

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Detalhes bibliográficos
Autores:	Silva, Alexandre Rodrigues, Cardoso, Rita de Cássia Soares, Silva, Lúcia Daniel Machado da
Formato:	artículo
Estado:	Versión publicada
Fecha de publicación:	2006
País:	Brasil
Recursos:	Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
Repositorio:	Brazilian Journal of Veterinary Research and Animal Science
Idioma:	portugués
OAI Identifier:	oai:revistas.usp.br:article/26555
Acesso em linha:	https://www.revistas.usp.br/bjvras/article/view/26555
Access Level:	acceso abierto
Palavra-chave:	Criopreservação Sêmen Cão Tris Água de coco Cryopreservation Dog Coconut water

id	BR_7ce73c98e03d1fec0cdea2f5f3cc5bcc
oai_identifier_str	oai:revistas.usp.br:article/26555
network_acronym_str	BR
network_name_str	Brasil
repository_id_str
dc.title.none.fl_str_mv	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation Comparação entre a água de coco em pó (ACP®) e o Tris como diluidores na criopreservação do sêmen de cães
title	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation
spellingShingle	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation Silva, Alexandre Rodrigues Criopreservação Sêmen Cão Tris Água de coco Cryopreservation Sêmen Dog Tris Coconut water
title_short	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation
title_full	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation
title_fullStr	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation
title_full_unstemmed	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation
title_sort	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation
dc.creator.none.fl_str_mv	Silva, Alexandre Rodrigues Cardoso, Rita de Cássia Soares Silva, Lúcia Daniel Machado da
author	Silva, Alexandre Rodrigues
author_facet	Silva, Alexandre Rodrigues Cardoso, Rita de Cássia Soares Silva, Lúcia Daniel Machado da
author_role	author
author2	Cardoso, Rita de Cássia Soares Silva, Lúcia Daniel Machado da
author2_role	author author
dc.subject.por.fl_str_mv	Criopreservação Sêmen Cão Tris Água de coco Cryopreservation Sêmen Dog Tris Coconut water
topic	Criopreservação Sêmen Cão Tris Água de coco Cryopreservation Sêmen Dog Tris Coconut water
description	The present study compared powder coconut water (ACP®) and Tris extenders on canine semen cryopreservation by classic evaluation and thermoresistance test. Five stud dogs were submitted to two semen collections by digital manipulation. Sperm fractions were analyzed regarding to its color, volume, sperm concentration, motility, vigor and morphology. Semen samples were divided into two aliquots: the first one was extended in Tris and second one in ACP®. Both extenders contained 20% egg yolk. Samples were cooled to 5ºC, glycerol added (6%), packaged in 0.25mL straws, frozen in nitrogen vapors and finally stored in liquid nitrogen. One week later, thaw was performed at 38ºC per 1min in water bath and new evaluations of sperm motility, vigor and morphology were conducted. Samples were kept at 38ºC in water bath and evaluated at 15 and 30min after thaw. No significant difference was observed between extenders throughout cryopreservation or thawing procedures, as well as during thermoresistance test, concerning to characteristics evaluated. Tris and ACP® were efficient in conserving 50% mobile spermatozoa and 70% morphologically normal sperm after thaw. Thus, ACP® can be used as an alternative extender for canine semen cryopreservation.
publishDate	2006
dc.date.none.fl_str_mv	2006-12-01
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	https://www.revistas.usp.br/bjvras/article/view/26555 10.11606/issn.1678-4456.bjvras.2006.26555
url	https://www.revistas.usp.br/bjvras/article/view/26555
identifier_str_mv	10.11606/issn.1678-4456.bjvras.2006.26555
dc.language.iso.fl_str_mv	por
language	por
dc.relation.none.fl_str_mv	https://www.revistas.usp.br/bjvras/article/view/26555/28338
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv	Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv	Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 6 (2006); 767-774 Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 6 (2006); 767-774 Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 6 (2006); 767-774 Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 6 (2006); 767-774 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP
instname_str	Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str	USP
institution	USP
reponame_str	Brazilian Journal of Veterinary Research and Animal Science
collection	Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv	Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv	bjvras@usp.br
_version_	1853662137676726272
spelling	Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservationComparação entre a água de coco em pó (ACP®) e o Tris como diluidores na criopreservação do sêmen de cãesCriopreservaçãoSêmenCãoTrisÁgua de cocoCryopreservationSêmenDogTrisCoconut waterThe present study compared powder coconut water (ACP®) and Tris extenders on canine semen cryopreservation by classic evaluation and thermoresistance test. Five stud dogs were submitted to two semen collections by digital manipulation. Sperm fractions were analyzed regarding to its color, volume, sperm concentration, motility, vigor and morphology. Semen samples were divided into two aliquots: the first one was extended in Tris and second one in ACP®. Both extenders contained 20% egg yolk. Samples were cooled to 5ºC, glycerol added (6%), packaged in 0.25mL straws, frozen in nitrogen vapors and finally stored in liquid nitrogen. One week later, thaw was performed at 38ºC per 1min in water bath and new evaluations of sperm motility, vigor and morphology were conducted. Samples were kept at 38ºC in water bath and evaluated at 15 and 30min after thaw. No significant difference was observed between extenders throughout cryopreservation or thawing procedures, as well as during thermoresistance test, concerning to characteristics evaluated. Tris and ACP® were efficient in conserving 50% mobile spermatozoa and 70% morphologically normal sperm after thaw. Thus, ACP® can be used as an alternative extender for canine semen cryopreservation.O presente trabalho comparou a água de coco em pó (ACP®) com o diluidor Tris na criopreservação do sêmen canino através da avaliação clássica e do teste de termorresistência. Cinco reprodutores caninos foram submetidos a duas coletas de sêmen através de manipulação digital. As frações espermáticas foram avaliadas quanto à sua coloração, volume, concentração, motilidade, vigor e morfologia espermática. As amostras de sêmen foram divididas em duas alíquotas: a primeira foi diluída em Tris e a segunda em ACP®. Ambos os diluidores continham 20% de gema de ovo. As amostras foram refrigeradas, adicionadas de glicerol (6%), envasadas em palhetas de 0,25mL, criopreservadas em vapores de nitrogênio, e finalmente armazenadas em nitrogênio líquido. Após uma semana, foi realizada a descongelação a 38ºC por 1min em banho-maria, sendo realizadas novas avaliações de motilidade, vigor e morfologia espermática. As amostras foram mantidas a 38ºC no banho-maria e avaliadas aos 15 e 30 min após descongelação. Não foram observadas diferenças significativas entre os dois diluidores no decorrer dos procedimentos de criopreservação e descongelação, bem como durante o teste de termorresistência, em relação às características avaliadas. O Tris e o ACP® foram eficientes em conservar 50% de espermatozóides móveis e 70% de espermatozóides morfologicamente normais após a descongelação. Assim, o ACP® pode ser utilizado como diluidor alternativo para a criopreservação do sêmen canino.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2655510.11606/issn.1678-4456.bjvras.2006.26555Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 Núm. 6 (2006); 767-774Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 No. 6 (2006); 767-774Brazilian Journal of Veterinary Research and Animal Science; v. 43 n. 6 (2006); 767-774Brazilian Journal of Veterinary Research and Animal Science; V. 43 N. 6 (2006); 767-7741678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26555/28338info:eu-repo/semantics/openAccessSilva, Alexandre RodriguesCardoso, Rita de Cássia SoaresSilva, Lúcia Daniel Machado da2020-06-23T04:16:31Zoai:revistas.usp.br:article/26555Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:50.016055Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
score	15.301629

Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation

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