Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela

IDAMS Clinical Study Group.

Detalhes bibliográficos
Autores: Morales, Ivonne, Rosenberger, Kerstin D., Magalhaes, Tereza, Morais, Clarice N. L., Braga, Cynthia, Marques, Ernesto T. A., Calvet, Guilherme Amaral, Damasceno, Luana, Brasil, Patrícia, Filippis, Ana Maria Bispo de, Tami, Adriana, Bethencourt, Sarah, Alvarez, Mayling, Martínez, Pedro A., Guzman, Maria G., Benevides, Bruno Souza, Caprara, Andrea, Quyen, Nguyen Than Ha, Simmons, Cameron P., Wills, Bridget, Lamballerie, Xavier de, Drexler, Jan Felix, Jaenisch, Thomas
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:Brasil
Recursos:Fundação Oswaldo Cruz (FIOCRUZ)
Repositório:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglês
OAI Identifier:oai:arca.fiocruz.br:icict/47856
Acesso em linha:https://arca.fiocruz.br/handle/icict/47856
Access Level:Acceso aberto
Palavra-chave:Zika virus
Enzyme-linked immunoassays
Dengue Virus
Chikungunya virus
Antibodies
Immunoassays
Serology
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dc.title.none.fl_str_mv Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
title Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
spellingShingle Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
Morales, Ivonne
Zika virus
Enzyme-linked immunoassays
Dengue Virus
Chikungunya virus
Antibodies
Immunoassays
Serology
title_short Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
title_full Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
title_fullStr Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
title_full_unstemmed Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
title_sort Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela
dc.creator.none.fl_str_mv Morales, Ivonne
Rosenberger, Kerstin D.
Magalhaes, Tereza
Morais, Clarice N. L.
Braga, Cynthia
Marques, Ernesto T. A.
Calvet, Guilherme Amaral
Damasceno, Luana
Brasil, Patrícia
Filippis, Ana Maria Bispo de
Tami, Adriana
Bethencourt, Sarah
Alvarez, Mayling
Martínez, Pedro A.
Guzman, Maria G.
Benevides, Bruno Souza
Caprara, Andrea
Quyen, Nguyen Than Ha
Simmons, Cameron P.
Wills, Bridget
Lamballerie, Xavier de
Drexler, Jan Felix
Jaenisch, Thomas
author Morales, Ivonne
author_facet Morales, Ivonne
Rosenberger, Kerstin D.
Magalhaes, Tereza
Morais, Clarice N. L.
Braga, Cynthia
Marques, Ernesto T. A.
Calvet, Guilherme Amaral
Damasceno, Luana
Brasil, Patrícia
Filippis, Ana Maria Bispo de
Tami, Adriana
Bethencourt, Sarah
Alvarez, Mayling
Martínez, Pedro A.
Guzman, Maria G.
Benevides, Bruno Souza
Caprara, Andrea
Quyen, Nguyen Than Ha
Simmons, Cameron P.
Wills, Bridget
Lamballerie, Xavier de
Drexler, Jan Felix
Jaenisch, Thomas
author_role author
author2 Rosenberger, Kerstin D.
Magalhaes, Tereza
Morais, Clarice N. L.
Braga, Cynthia
Marques, Ernesto T. A.
Calvet, Guilherme Amaral
Damasceno, Luana
Brasil, Patrícia
Filippis, Ana Maria Bispo de
Tami, Adriana
Bethencourt, Sarah
Alvarez, Mayling
Martínez, Pedro A.
Guzman, Maria G.
Benevides, Bruno Souza
Caprara, Andrea
Quyen, Nguyen Than Ha
Simmons, Cameron P.
Wills, Bridget
Lamballerie, Xavier de
Drexler, Jan Felix
Jaenisch, Thomas
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.en.none.fl_str_mv Zika virus
Enzyme-linked immunoassays
Dengue Virus
Chikungunya virus
Antibodies
Immunoassays
Serology
topic Zika virus
Enzyme-linked immunoassays
Dengue Virus
Chikungunya virus
Antibodies
Immunoassays
Serology
description IDAMS Clinical Study Group.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-24T13:46:39Z
dc.date.available.fl_str_mv 2021-06-24T13:46:39Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv MORALES, Ivonne et al. Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela. PLoS Neglected Tropical diseases, v. 15, n. 4, p. 1-19, Apr. 2021.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/47856
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0009336
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv MORALES, Ivonne et al. Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela. PLoS Neglected Tropical diseases, v. 15, n. 4, p. 1-19, Apr. 2021.
1935-2727
10.1371/journal.pntd.0009336
1935-2735
url https://arca.fiocruz.br/handle/icict/47856
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2021-06-24T13:46:39Z2021-06-24T13:46:39Z2021MORALES, Ivonne et al. Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela. PLoS Neglected Tropical diseases, v. 15, n. 4, p. 1-19, Apr. 2021.1935-2727https://arca.fiocruz.br/handle/icict/4785610.1371/journal.pntd.00093361935-2735IDAMS Clinical Study Group.This study was funded by the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 281803, IDAMS (http://www.idams.eu/). In addition, the work was supported by the European Union’s Horizon 2020 research and innovation programme (ZIKAlliance grant agreement no. 734548, https://zikalliance.tghn.org/). The work at the Fiocruz Flavivirus Laboratory was supported by Faperj under the grant no. E-26/202.930/2016 and by the Brazilian Ministry of Health. Approximately 50% of the test kits were provided free of charge by Euroimmun, while the other 50% were purchased by the researchers. The funders or Euroimmun had no influence on the study design, the testing, or the data analysis, decision to publish, or preparation of the manuscript at any point in time.Heidelberg University Hospital. Department for Infectious Diseases. Section Clinical Tropical Medicine. Heidelberg, Germany / Associated partner Heidelberg University Hospital. German Centre for Infection Research. Heidelberg, Germany.Heidelberg University Hospital. Department for Infectious Diseases. Section Clinical Tropical Medicine. Heidelberg, Germany / Associated partner Heidelberg University Hospital. German Centre for Infection Research. Heidelberg, Germany.Colorado State University. Department of Microbiology, Immunology and Pathology. Center for Vector-Borne Infectious Diseases. Fort Collins, Colorado, USA.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapêutica Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil / Instituto de Medicina Integral Professor Fernando Figueira. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapêutica Experimental. Recife, PE, Brasil / University of Pittsburgh. Graduate School of Public Health. Department of Infectious Diseases. Pittsburgh, Pennsylvania, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ, Brasil.University of Groningen. University Medical Center Groningen. Department of Medical Microbiology and Infection Prevention. Groningen, The Netherlands / Universidad de Carabobo. Facultad de Ciencias de la Salud. Valencia, Venezuela.Universidad de Carabobo. Facultad de Ciencias de la Salud. Valencia, Venezuela.Instituto de Medicina Tropical Pedro Kouri. La Habana, Cuba.Instituto de Medicina Tropical Pedro Kouri. La Habana, Cuba.Instituto de Medicina Tropical Pedro Kouri. La Habana, Cuba.Universidade Estadual do Ceará. Fortaleza, CE, Brasil.Universidade Estadual do Ceará. Fortaleza, CE, Brasil.Oxford University Clinical Research Unit. Hospital for Tropical Diseases. Ho Chi Minh City, Vietnam.Oxford University Clinical Research Unit. Hospital for Tropical Diseases. Ho Chi Minh City, Vietnam / Monash University. Institute for Vector-Borne Disease. Melbourne, Australia.Oxford University Clinical Research Unit. Hospital for Tropical Diseases. Ho Chi Minh City, Vietnam / Oxford University. Nuffield Department of Clinical Medicine. Centre for Tropical Medicine and Global Health. Oxford, United Kingdom.Unité des Virus Emergents. Marseille, France.Humboldt-Universität zu Berlin. Charité-Universitätsmedizin Berlin. Institute of Virology. Berlin, Germany / Sechenov University. Martsinovsky Institute of Medical Parasitology. Tropical and Vector-Borne Diseases. Moscow, Russia / Universitätsmedizin Berlin. German Centre for Infection Research. Berlin, Germany.Background: Serological diagnosis of Zika virus (ZIKV) infection is challenging because of the antibody cross-reactivity among flaviviruses. At the same time, the role of Nucleic Acid Testing (NAT) is limited by the low proportion of symptomatic infections and the low average viral load. Here, we compared the diagnostic performance of commercially available IgM, IgAM, and IgG ELISAs in sequential samples during the ZIKV and chikungunya (CHIKV) epidemics and co-circulation of dengue virus (DENV) in Brazil and Venezuela. Methodology/principal findings: Acute (day of illness 1-5) and follow-up (day of illness ≥ 6) blood samples were collected from nine hundred and seven symptomatic patients enrolled in a prospective multicenter study between June 2012 and August 2016. Acute samples were tested by RT-PCR for ZIKV, DENV, and CHIKV. Acute and follow-up samples were tested for IgM, IgAM, and IgG antibodies to ZIKV using commercially available ELISAs. Among follow-up samples with a RT-PCR confirmed ZIKV infection, anti-ZIKV IgAM sensitivity was 93.5% (43/46), while IgM and IgG exhibited sensitivities of 30.3% (10/33) and 72% (18/25), respectively. An additional 24% (26/109) of ZIKV infections were detected via IgAM seroconversion in ZIKV/DENV/CHIKV RT-PCR negative patients. The specificity of anti-ZIKV IgM was estimated at 93% and that of IgAM at 85%. Conclusions/significance: Our findings exemplify the challenges of the assessment of test performance for ZIKV serological tests in the real-world setting, during co-circulation of DENV, ZIKV, and CHIKV. However, we can also demonstrate that the IgAM immunoassay exhibits superior sensitivity to detect ZIKV RT-PCR confirmed infections compared to IgG and IgM immunoassays. The IgAM assay also proves to be promising for detection of anti-ZIKV seroconversions in sequential samples, both in ZIKV PCR-positive as well as PCR-negative patients, making this a candidate assay for serological monitoring of pregnant women in future ZIKV outbreaks.engPublic Library of ScienceDiagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuelainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleZika virusEnzyme-linked immunoassaysDengue VirusChikungunya virusAntibodiesImmunoassaysSerologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZMorales, IvonneRosenberger, Kerstin D.Magalhaes, TerezaMorais, Clarice N. L.Braga, CynthiaMarques, Ernesto T. 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score 15.228081

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