Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique
Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope an...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1998 |
| País: | Brasil |
| Recursos: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/674 |
| Acesso em linha: | http://dx.doi.org/10.1590/S0100-879X1998000900007 http://repositorio.unifesp.br/handle/11600/674 |
| Access Level: | acceso abierto |
| Palavra-chave: | autoantibodies detection Addison's disease peroxidase-labelled protein A indirect immunofluorescence adrenal gland |
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Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A techniqueautoantibodies detectionAddison's diseaseperoxidase-labelled protein Aindirect immunofluorescenceadrenal glandAdrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera.Universidade Federal de São Paulo (UNIFESP)University of PerugiaUNIFESPSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)University of Perugia2015-06-14T13:24:46Z2015-06-14T13:24:46Z1998-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1141-1148application/pdfhttp://dx.doi.org/10.1590/S0100-879X1998000900007Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 9, p. 1141-1148, 1998.10.1590/S0100-879X1998000900007S0100-879X1998000900007.pdf0100-879XS0100-879X1998000900007http://repositorio.unifesp.br/handle/11600/674WOS:000076297100007ark:/48912/0013000023x0zengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPSilva, Reinaldo Correia [UNIFESP]Faiçal, Samir [UNIFESP]Laureti, StefanoFalorni, AlbertoDib, Sergio Atala [UNIFESP]Kater, Claudio Elias [UNIFESP]2024-07-29T16:58:59Zoai:repositorio.unifesp.br:11600/674Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T16:58:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| spellingShingle |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique Silva, Reinaldo Correia [UNIFESP] autoantibodies detection Addison's disease peroxidase-labelled protein A indirect immunofluorescence adrenal gland |
| title_short |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_full |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_fullStr |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_full_unstemmed |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_sort |
Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| dc.creator.none.fl_str_mv |
Silva, Reinaldo Correia [UNIFESP] Faiçal, Samir [UNIFESP] Laureti, Stefano Falorni, Alberto Dib, Sergio Atala [UNIFESP] Kater, Claudio Elias [UNIFESP] |
| author |
Silva, Reinaldo Correia [UNIFESP] |
| author_facet |
Silva, Reinaldo Correia [UNIFESP] Faiçal, Samir [UNIFESP] Laureti, Stefano Falorni, Alberto Dib, Sergio Atala [UNIFESP] Kater, Claudio Elias [UNIFESP] |
| author_role |
author |
| author2 |
Faiçal, Samir [UNIFESP] Laureti, Stefano Falorni, Alberto Dib, Sergio Atala [UNIFESP] Kater, Claudio Elias [UNIFESP] |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) University of Perugia |
| dc.subject.por.fl_str_mv |
autoantibodies detection Addison's disease peroxidase-labelled protein A indirect immunofluorescence adrenal gland |
| topic |
autoantibodies detection Addison's disease peroxidase-labelled protein A indirect immunofluorescence adrenal gland |
| description |
Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera. |
| publishDate |
1998 |
| dc.date.none.fl_str_mv |
1998-09-01 2015-06-14T13:24:46Z 2015-06-14T13:24:46Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X1998000900007 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 9, p. 1141-1148, 1998. 10.1590/S0100-879X1998000900007 S0100-879X1998000900007.pdf 0100-879X S0100-879X1998000900007 http://repositorio.unifesp.br/handle/11600/674 WOS:000076297100007 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/0013000023x0z |
| url |
http://dx.doi.org/10.1590/S0100-879X1998000900007 http://repositorio.unifesp.br/handle/11600/674 |
| identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 9, p. 1141-1148, 1998. 10.1590/S0100-879X1998000900007 S0100-879X1998000900007.pdf 0100-879X S0100-879X1998000900007 WOS:000076297100007 ark:/48912/0013000023x0z |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Brazilian Journal of Medical and Biological Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
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1141-1148 application/pdf |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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