Cytogenetic analysis of A-, B-chromosomes and ZZ/ZW sex chromosomes of Characidium gomesi (Teleostei, Characiformes, Crenuchidae)

Different cytogenetic techniques were used to analyze the chromosomes of Characidium gomesi with the main objective of comparing the base composition of ZZ/ZW sex-chromosomes, B-chromosomes and the heterochromatin of A-chromosomes. The results of digestion of chromosomes with AluI restriction endonu...

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Detalles Bibliográficos
Autores: Maistro, Edson Luis, Jesus, Célia Maria de, Oliveira, Claudio, Moreira-Filho, Orlando, Foresti, Fausto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/67747
Acceso en línea:http://dx.doi.org/10.1508/cytologia.69.181
http://hdl.handle.net/11449/67747
Access Level:acceso abierto
Palabra clave:B-chromosomes
Characidium gomesi
Fish cytogenetics
Heterochromatin differentiation
ZZ/ZW sex chromosome system
Characidium
Characiformes
Crenuchidae
Teleostei
Descripción
Sumario:Different cytogenetic techniques were used to analyze the chromosomes of Characidium gomesi with the main objective of comparing the base composition of ZZ/ZW sex-chromosomes, B-chromosomes and the heterochromatin of A-chromosomes. The results of digestion of chromosomes with AluI restriction endonuclease (RE), silver and CMA3 staining, C-banding and fluorescence in situ hybridization (FISH) with the 18S rDNA probe suggested the existence of compositional differences between the heterochromatin of ZZ/ZW sex-chromosomes, A- and B-chromosomes, and indicated the presence of different numbers and morphology of B-chromosomes in the samples of this population.