Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis

In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, name...

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Detalhes bibliográficos
Autores: Calixto, Christiano, Silva, Jhenifer N., Sampaio, Angélica Fernandes Arcanjo, Nogueira, Cintia Lopes, Abreu, Leonardo Araujo de, Oliveira, Pedro Lagerblad de, Gondim, Katia Calp, Moraes, Bruno Marques Costa de, Carvalho, Stephanie Serafim de, Silva, Renato Martins da, Vaz Junior, Itabajara da Silva, Moreira, Luciano A., Logullo, Carlos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Recursos:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/231311
Acesso em linha:http://hdl.handle.net/10183/231311
Access Level:acceso abierto
Palavra-chave:Wolbachia
Metabolismo energético
Respostas imunes
Aedes fluviatilis
Linhagem celular
Interações hospedeiro-patógeno
Descrição
Resumo:In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.

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