Removal of phenol by enzymatic oxidation and flotation

This work presents a process for phenol removal comprising a reaction step in which phenol is polymerized in the presence of an enzyme followed by a separation step involving dissolved air flotation (DAF). A crude preparation from horseradish roots was used as a low purity source of the enzyme horse...

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Detalhes bibliográficos
Autores: Wilberg, Katia de Quadros, Nunes, Denise Goulart, Rubio, Jorge
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Brasil
Recursos:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/75858
Acesso em linha:http://hdl.handle.net/10183/75858
Access Level:acceso abierto
Palavra-chave:Flotação
Tratamento de minérios
Phenol removal
Horseradish peroxidase
Flotation
Descrição
Resumo:This work presents a process for phenol removal comprising a reaction step in which phenol is polymerized in the presence of an enzyme followed by a separation step involving dissolved air flotation (DAF). A crude preparation from horseradish roots was used as a low purity source of the enzyme horseradish peroxidase (HRP). The technical feasibility of the process was studied at bench scale using 1 to 10 mM synthetic phenol solutions. Experimental results showed the potential of the proposed technique. A phenol conversion higher than 99 % was observed at the polymerization step and an efficiency higher than 94 % was achieved at the separation stage. Despite the use of a low purity source of HRP, which increases the input of organic matter, the chemical oxygen demand (COD) decreased by 50 %.