Ostras, Crassostrea rhizophorae, contaminadas con Vibrio parahaemolyticus Kanagawa positivas

The aim of the present research was to confirm the phenotypic identification of Vibrio parahaemolyticus strains isolated from two markets of fresh and frozen oysters on a beach at the Northeastern of Brazil by molecular biology testes. In order to achieve this goal, phenotypic identification was per...

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Detalles Bibliográficos
Autores: Menezes, Francisca Gleire Rodrigues de, Araújo, Rayza Lima, Rodrigues, Marina Teresa Torres, Rocha, Rafael dos Santos, Sousa, Oscarina Viana de, Vieira, Regine Helena Silva dos Fernandes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:español
OAI Identifier:oai:repositorio.ufc.br:riufc/67262
Acceso en línea:http://www.repositorio.ufc.br/handle/riufc/67262
Access Level:acceso abierto
Palabra clave:Bivalves
Speciation - Vibrio parahaemolyticus
Virulence
Bivalvos
Especiação - Vibrio parahaemolyticus
Virulência
Descripción
Sumario:The aim of the present research was to confirm the phenotypic identification of Vibrio parahaemolyticus strains isolated from two markets of fresh and frozen oysters on a beach at the Northeastern of Brazil by molecular biology testes. In order to achieve this goal, phenotypic identification was performed by dichotomous key, while genotypic tests were performed by detection of gene vib, to confirmation of genus Vibrio, and gene tl, to specie V. parahaemolyticus. Also, the virulence potential of strains was investigated by Kanagawa and urease tests. Of the 15 tested strains, 14 were confirmed to genus Vibrio and specie V. parahaemolyticus. Twelve (86%) strains were positive to Kanagawa test; however no strains were positive to urease test. Thus, we concluded that the dichotomous key was efficient in the identification of V. parahaemolyticus and the marketed oysters are able of transmitting gastroenteritis to consumer if they are eaten raw.