Fractionation and identification of the nucleic acid-related substances secreted by Streptomyces aureofaciens

S. aureofaciens growth in a chemically defined medium was associated with the active secretion of nucleic acid-related substances in the medium. High secretion depended on low availability of phosphate, and fractionation showed 7 anionic substances were secreted as major components. When compared to...

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Detalhes bibliográficos
Autores: De Carvalho, Alírio [UNESP], Malavolta, Cristina Marques [UNESP], Quirino, Gustavo Hoff [UNESP], Fernandes, José Atanasio Dos Santos [UNESP], Mengatti, Jair [UNESP], Contiero, Jonas [UNESP], Oppermann, Heloísa Ribi [UNESP], Molinari, Rubens [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:1996
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/224609
Acesso em linha:http://hdl.handle.net/11449/224609
Access Level:acceso abierto
Palavra-chave:Fractionation and identification
Nitrogenous bases
Nucleic acid-related substances
Nucleosides
Streptomyces aureofaciens
Descrição
Resumo:S. aureofaciens growth in a chemically defined medium was associated with the active secretion of nucleic acid-related substances in the medium. High secretion depended on low availability of phosphate, and fractionation showed 7 anionic substances were secreted as major components. When compared to 76 known purine and pyrimidine derivatives only orotic acid was identified. Cationic components are among the minor concentration components secreted which have been identified as cytosine, inosine, cytidine, adenine, guanine and, probably, 1-methyl-adenine.