Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs

Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.

Detalhes bibliográficos
Autores: Porrozzi, Renato, Costa, Marcos V. Santos da, Teva, Antonio, Falqueto, Aloísio, Ferreira, Adelson L., Santos, Claudiney D. dos, Fernandes, Ana Paula, Gazzinelli, Ricardo T., Campos Neto, Antonio, Grimaldi Jr., Gabriel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2007
País:Brasil
Recursos:Fundação Oswaldo Cruz (FIOCRUZ)
Repositório:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglês
OAI Identifier:oai:arca.fiocruz.br:icict/19323
Acesso em linha:https://arca.fiocruz.br/handle/icict/19323
Access Level:Acceso aberto
Palavra-chave:Cães
Infecções viscerais
Leishmania infantum
Sorodiagnóstico
Imunossorvente ligado a enzimas
Antígenos
Dogs
Visceral Infections
Serodiagnosis
Enzyme-Linked Immunosorbent
Antigens
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repository_id_str
dc.title.none.fl_str_mv Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
title Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
spellingShingle Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
Porrozzi, Renato
Cães
Infecções viscerais
Leishmania infantum
Sorodiagnóstico
Imunossorvente ligado a enzimas
Antígenos
Leishmania infantum
Dogs
Visceral Infections
Serodiagnosis
Enzyme-Linked Immunosorbent
Antigens
title_short Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
title_full Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
title_fullStr Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
title_full_unstemmed Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
title_sort Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
dc.creator.none.fl_str_mv Porrozzi, Renato
Costa, Marcos V. Santos da
Teva, Antonio
Falqueto, Aloísio
Ferreira, Adelson L.
Santos, Claudiney D. dos
Fernandes, Ana Paula
Gazzinelli, Ricardo T.
Campos Neto, Antonio
Grimaldi Jr., Gabriel
author Porrozzi, Renato
author_facet Porrozzi, Renato
Costa, Marcos V. Santos da
Teva, Antonio
Falqueto, Aloísio
Ferreira, Adelson L.
Santos, Claudiney D. dos
Fernandes, Ana Paula
Gazzinelli, Ricardo T.
Campos Neto, Antonio
Grimaldi Jr., Gabriel
author_role author
author2 Costa, Marcos V. Santos da
Teva, Antonio
Falqueto, Aloísio
Ferreira, Adelson L.
Santos, Claudiney D. dos
Fernandes, Ana Paula
Gazzinelli, Ricardo T.
Campos Neto, Antonio
Grimaldi Jr., Gabriel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Cães
Infecções viscerais
Leishmania infantum
Sorodiagnóstico
Imunossorvente ligado a enzimas
Antígenos
topic Cães
Infecções viscerais
Leishmania infantum
Sorodiagnóstico
Imunossorvente ligado a enzimas
Antígenos
Leishmania infantum
Dogs
Visceral Infections
Serodiagnosis
Enzyme-Linked Immunosorbent
Antigens
dc.subject.en.none.fl_str_mv Leishmania infantum
Dogs
Visceral Infections
Serodiagnosis
Enzyme-Linked Immunosorbent
Antigens
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2017-06-06T15:32:54Z
dc.date.available.fl_str_mv 2017-06-06T15:32:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PORROZZI, Renato; et al. Comparative Evaluation of Enzyme-Linked Immunosorbent Assays Based on Crude and Recombinant Leishmanial Antigens for Serodiagnosis of Symptomatic and Asymptomatic Leishmania infantum Visceral Infections in Dogs. Clinical and Vaccine Immunology, v.14, n.5, p.544-548, May 2007.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/19323
dc.identifier.issn.none.fl_str_mv 1556-6811
dc.identifier.doi.none.fl_str_mv 10.1128/CVI.00420-06
dc.identifier.eissn.none.fl_str_mv 1556-679X
identifier_str_mv PORROZZI, Renato; et al. Comparative Evaluation of Enzyme-Linked Immunosorbent Assays Based on Crude and Recombinant Leishmanial Antigens for Serodiagnosis of Symptomatic and Asymptomatic Leishmania infantum Visceral Infections in Dogs. Clinical and Vaccine Immunology, v.14, n.5, p.544-548, May 2007.
1556-6811
10.1128/CVI.00420-06
1556-679X
url https://arca.fiocruz.br/handle/icict/19323
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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spelling 2017-06-06T15:32:54Z2017-06-06T15:32:54Z2007PORROZZI, Renato; et al. Comparative Evaluation of Enzyme-Linked Immunosorbent Assays Based on Crude and Recombinant Leishmanial Antigens for Serodiagnosis of Symptomatic and Asymptomatic Leishmania infantum Visceral Infections in Dogs. Clinical and Vaccine Immunology, v.14, n.5, p.544-548, May 2007.1556-6811https://arca.fiocruz.br/handle/icict/1932310.1128/CVI.00420-061556-679XengAmerican Society for MicrobiologyCãesInfecções visceraisLeishmania infantumSorodiagnósticoImunossorvente ligado a enzimasAntígenosLeishmania infantumDogsVisceral InfectionsSerodiagnosisEnzyme-Linked ImmunosorbentAntigensComparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal do Espírito Santo. Vitória, ES, Brasil.,Universidade Federal do Espírito Santo. Vitória, ES, Brasil.,Universidade Federal do Espírito Santo. Vitória, ES, Brasil.,Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.The Forsyth Institute. Boston, Massachusetts, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.The diagnosis of visceral leishmaniasis remains difficult in rural areas where the disease is endemic, and serologic methods still need assessment, as they are not very sensitive for the detection of asymptomatic infectious dogs. Here we present data on the development of enzyme-linked immunosorbent assay (ELISA)-based methods for the detection of antibodies against recombinant leishmanial antigens (namely, the recombinant K26 [rK26] and rK39 antigens from Leishmania infantum and the rA2 protein from Leishmania donovani) in comparison to ELISAs employing crude soluble antigen (CSA). The assays utilized sera from known negative controls (n=25) and clinically asymptomatic (n=50) and symptomatic (n=50) dogs with confirmed L. infantum infections. Additional studies were also done using sera from animals harboring other infections (n=14) for the evaluation of cross-reactivity. Our study indicated that rK26 and rK39 used in ELISAs provided very high sensitivities for the detection of symptomatic dogs (94% and 100%, respectively), followed by CSA (88%) and rA2 (70%). Conversely, rA2 was more sensitive for asymptomatic dogs (88%) than rK39 and rK26 (both 66%) and CSA (30%). Some cross-reactivity in sera from dogs with other infections (Leishmania braziliensis and Leptospira interrogans) was identified, but the rA2 protein provided the greatest specificity (98%). Data further indicate that all three recombinant proteins must be used in parallel to detect essentially all infected dogs. Efforts should be made to develop a cheap and reliable serologic test based on epitope selection from these diagnostic markers for the sensitive detection of L. infantum-infected dogs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZPorrozzi, RenatoCosta, Marcos V. 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