Comparação de dois métodos de extração de Xanthomonas campestris pv. phaseoli em sementes de feijoeiro
The objective of this research was to evaluate the effectiveness of the extraction of Xanthomonas campestris pv. phaseoli from naturally infected dry bean seeds. Extraction methods tested included soaking whole seeds in sterilized saline phosphate buffer and crushing seeds after soaking in sterilize...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 1998 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositório: | Repositório Institucional da UNESP |
| Idioma: | português |
| OAI Identifier: | oai:repositorio.unesp.br:11449/65440 |
| Acesso em linha: | http://seer.sct.embrapa.br/index.php/pab/article/view/4876 http://hdl.handle.net/11449/65440 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Common bacterial blight Phaseolus vulgaris Seed pathology Bacteria (microorganisms) Phaseolus (angiosperm) Xanthomonas Xanthomonas campestris pv. phaseoli |
| Resumo: | The objective of this research was to evaluate the effectiveness of the extraction of Xanthomonas campestris pv. phaseoli from naturally infected dry bean seeds. Extraction methods tested included soaking whole seeds in sterilized saline phosphate buffer and crushing seeds after soaking in sterilized saline phosphate buffer. The bacterium was isolated on a semiselective agar medium. The seed crushing method was found to be more effective. The bacterium strains isolated were pathogenic to bean leaves, reacted with X. campestris pv. phaseoli antiserum, and had morphological and physiological/biochemical characteristics typical of the X. campestris pv. phaseoli. |
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