Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis

NIH-NIAID grant # AI-68835, AI-7712375, and AI-097830 to KC. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) – (Programa Visitante Especial) Processo 402312/2012-0 to CdO. SV, FC, and LR were supported by CNPq fellowships and CdO is a sênior investigator for CNPq.

Detalhes bibliográficos
Autores: Viana, Sayonara M., Celes, Fabiana Santana, Ramirez, Laura, Kolli, Bala, Ng, Dennis K. P., Chang, Kwang P., Oliveira, Camila Indiani de
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2018
País:Brasil
Recursos:Fundação Oswaldo Cruz (FIOCRUZ)
Repositório:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglês
OAI Identifier:oai:arca.fiocruz.br:icict/25875
Acesso em linha:https://arca.fiocruz.br/handle/icict/25875
Access Level:Acceso aberto
Palavra-chave:Leishmania
Leishmaniose
Potossensibilizador
Ftalocianina
Uroporfirina
Vacinação fotodinâmica
Vacinação suicida
Leishmaniose cutânea
Leishmaniasis
Potosensitizer
Phthalocyanine
Uroporphyrin
Photodynamic vaccination
Suicidal vaccination
Cutaneous leishmaniasis
03 Saúde e Bem-Estar
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dc.title.none.fl_str_mv Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
title Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
spellingShingle Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
Viana, Sayonara M.
Leishmania
Leishmaniose
Potossensibilizador
Ftalocianina
Uroporfirina
Vacinação fotodinâmica
Vacinação suicida
Leishmaniose cutânea
Leishmania
Leishmaniasis
Potosensitizer
Phthalocyanine
Uroporphyrin
Photodynamic vaccination
Suicidal vaccination
Cutaneous leishmaniasis
03 Saúde e Bem-Estar
title_short Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
title_full Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
title_fullStr Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
title_full_unstemmed Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
title_sort Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis
dc.creator.none.fl_str_mv Viana, Sayonara M.
Celes, Fabiana Santana
Ramirez, Laura
Kolli, Bala
Ng, Dennis K. P.
Chang, Kwang P.
Oliveira, Camila Indiani de
author Viana, Sayonara M.
author_facet Viana, Sayonara M.
Celes, Fabiana Santana
Ramirez, Laura
Kolli, Bala
Ng, Dennis K. P.
Chang, Kwang P.
Oliveira, Camila Indiani de
author_role author
author2 Celes, Fabiana Santana
Ramirez, Laura
Kolli, Bala
Ng, Dennis K. P.
Chang, Kwang P.
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Leishmania
Leishmaniose
Potossensibilizador
Ftalocianina
Uroporfirina
Vacinação fotodinâmica
Vacinação suicida
Leishmaniose cutânea
topic Leishmania
Leishmaniose
Potossensibilizador
Ftalocianina
Uroporfirina
Vacinação fotodinâmica
Vacinação suicida
Leishmaniose cutânea
Leishmania
Leishmaniasis
Potosensitizer
Phthalocyanine
Uroporphyrin
Photodynamic vaccination
Suicidal vaccination
Cutaneous leishmaniasis
03 Saúde e Bem-Estar
dc.subject.en.none.fl_str_mv Leishmania
Leishmaniasis
Potosensitizer
Phthalocyanine
Uroporphyrin
Photodynamic vaccination
Suicidal vaccination
Cutaneous leishmaniasis
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
description NIH-NIAID grant # AI-68835, AI-7712375, and AI-097830 to KC. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) – (Programa Visitante Especial) Processo 402312/2012-0 to CdO. SV, FC, and LR were supported by CNPq fellowships and CdO is a sênior investigator for CNPq.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-04-16T19:19:02Z
dc.date.available.fl_str_mv 2018-04-16T19:19:02Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIANA, Sayonara M. et al. Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis. Frontiers in Microbiology, v. 9, p. 1-10, 2018.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/25875
dc.identifier.issn.none.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2018.00165
identifier_str_mv VIANA, Sayonara M. et al. Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis. Frontiers in Microbiology, v. 9, p. 1-10, 2018.
1664-302X
10.3389/fmicb.2018.00165
url https://arca.fiocruz.br/handle/icict/25875
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2018-04-16T19:19:02Z2018-04-16T19:19:02Z2018VIANA, Sayonara M. et al. Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis. Frontiers in Microbiology, v. 9, p. 1-10, 2018.1664-302Xhttps://arca.fiocruz.br/handle/icict/2587510.3389/fmicb.2018.00165NIH-NIAID grant # AI-68835, AI-7712375, and AI-097830 to KC. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) – (Programa Visitante Especial) Processo 402312/2012-0 to CdO. SV, FC, and LR were supported by CNPq fellowships and CdO is a sênior investigator for CNPq.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Rosalind Franklin University of Medicine and Science. Chicago Medical School. Department of Microbiology/Immunology. North Chicago, IL, USA.The Chinese University of Hong Kong. Department of Chemistry. Hong Kong, Hong Kong.Rosalind Franklin University of Medicine and Science. Chicago Medical School. Department of Microbiology/Immunology. North Chicago, IL, USA.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Background: Photosensitizers (PS), like porphyrins and phthalocyanines (PC) are excitable by light to generate cytotoxic singlet oxygen and other reactive oxygen species in the presence of atmospheric O2. Photodynamic inactivation of Leishmania by this means renders them non-viable, but preserves their effective use as vaccines. Leishmania can be photo-inactivated after PS-sensitization by loading via their endocytic uptake of PC or endogenous induction of transgenic mutants with delta-aminolevulinate (ALA) to accumulate cytosolic uroporphyrin I (URO). Here, PS-sensitization and photo-inactivation of Leishmaniaamazonensis was further examined in vitro and in vivo for vaccination against cutaneous leishmaniasis (CL). Methods and Results:Leishmania amazonensis promastigotes were photodynamically inactivated in vitro by PC-loading followed by exposure to red light (1-2 J/cm2) or ALA-induction of uroporphyrinogenic transfectants to accumulate cytosolic URO followed by longwave UV exposure. When applied individually, both strategies of photodynamic inactivation were found to significantly, albeit incompletely abolish the MTT reduction activities of the promastigotes, their uptake by mouse bone marrow-derived macrophages in vitro and their infectivity to mouse ear dermis in vivo. Inactivation of Leishmania to completion by using a combination of both strategies was thus used for the sake of safety as whole-cell vaccines for immunization of BALB/c mice. Different cutaneous sites were assessed for the efficacy of such photodynamic vaccination in vivo. Each site was inoculated first with in vitro doubly PS-sensitized promastigotes and then spot-illuminated with white light (50 J/cm2) for their photo-inactivation in situ. Only in ear dermis parasites were photo-inactivated beyond detection. Mice were thus immunized once in the ear and challenged 3 weeks later at the tail base with virulent L. amazonensis. Prophylaxis was noted in mice photodynamically vaccinated with doubly photo-inactivated parasites, as indicated by a significant delay in the onset of lesion development and a substantial decrease in the parasite loads. Conclusion: Leishmania doubly PS-sensitized and in situ photo-inactivated as described proved to be safe and effective when used for one-time immunization of ear dermis, as indicated by its significant protection of the inherently very susceptible BALB/c mice against CL.engFrontiers MediaLeishmaniaLeishmaniosePotossensibilizadorFtalocianinaUroporfirinaVacinação fotodinâmicaVacinação suicidaLeishmaniose cutâneaLeishmaniaLeishmaniasisPotosensitizerPhthalocyanineUroporphyrinPhotodynamic vaccinationSuicidal vaccinationCutaneous leishmaniasis03 Saúde e Bem-EstarPhotodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZViana, Sayonara M.Celes, Fabiana SantanaRamirez, LauraKolli, BalaNg, Dennis K. P.Chang, Kwang P.Oliveira, Camila Indiani deLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/1b61d6db-5999-45d7-83d4-654b739bf4f8/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALViana S Photodynamic vaccination of BALBc mice ....pdfViana S Photodynamic vaccination of BALBc mice ....pdfapplication/pdf1972289https://arca.fiocruz.br/bitstreams/7a87971b-61e7-46e7-8a6e-677f4ff7ce72/downloadd8f1288efe88a2a888d4aea1d0c76945MD52trueAnonymousREADTEXTViana S Photodynamic vaccination of BALBc mice ....pdf.txtViana S Photodynamic vaccination of BALBc mice ....pdf.txtExtracted texttext/plain58833https://arca.fiocruz.br/bitstreams/9f08d778-3c01-4551-beab-84597f036994/downloadc601cb6003e89306d020cfa0cc82bd41MD57falseAnonymousREADTHUMBNAILViana S Photodynamic vaccination of BALBc mice ....pdf.jpgViana S Photodynamic vaccination of BALBc mice ....pdf.jpgGenerated Thumbnailimage/jpeg41112https://arca.fiocruz.br/bitstreams/f4864cd3-5403-461c-8357-431f23c69b61/download36a21bd41aa2c7e579999b112b4f8482MD58falseAnonymousREADicict/258752025-12-10 14:30:31.801open.accessoai:arca.fiocruz.br:icict/25875https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T17:30:31Repositório Institucional da FIOCRUZ (ARCA) - 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