Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.

Souza, Ronny Francisco de; Santos, Yaro Luciolo dos; Vasconcellos, Raphael de Souza; Pereira, Lucas Borges; Caldas, Ivo Santana; Almeida, Márcia Rogéria de; Bahia, Maria Terezinha; Fietto, Juliana Lopes Rangel

Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.

Canine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to hu...

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Autores:	Souza, Ronny Francisco de, Santos, Yaro Luciolo dos, Vasconcellos, Raphael de Souza, Pereira, Lucas Borges, Caldas, Ivo Santana, Almeida, Márcia Rogéria de, Bahia, Maria Terezinha, Fietto, Juliana Lopes Rangel
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2013
País:	Brasil
Institución:	Universidade Federal de Ouro Preto (UFOP)
Repositorio:	Repositório Institucional da UFOP
Idioma:	inglés
OAI Identifier:	oai:repositorio.ufop.br:123456789/4928
Acceso en línea:	http://www.repositorio.ufop.br/handle/123456789/4928 https://doi.org/10.1016/j.actatropica.2012.09.011
Access Level:	acceso abierto
Palabra clave:	Canine leishmaniasis Immunodiagnosis Recombinant protein Ecto nucleoside triphosphate Diphosphohydrolase

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spelling	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.Canine leishmaniasisImmunodiagnosisRecombinant proteinEcto nucleoside triphosphateDiphosphohydrolaseCanine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection worldwide. Leishmania (Leishmania) infantum (L. chagasi) is the etiologic agent of visceral leishmaniasis in the New World. In this work, a new L. (L.) infantum (L. chagasi) recombinant antigen, named ATP diphosphohydrolase (rLic-NTPDase-2), intended for use in the immunodiagnosis of CVL was produced and validated. The extracellular domain of ATP diphosphohydrolase was cloned and expressed in the pET21b-Escherichia coli expression system. Indirect ELISA assays were used to detect the purified rLic-NTPDase-2 antigen using a standard canine sera library. This library contained CVL-positive samples, leishmaniasis-negative samples and samples from Trypanosoma cruzi-infected dogs. The results show a high sensitivity of 100% (95% CI = 92.60–100.0%) and a high specificity of 100% (95% CI = 86.77–100.0%), with a high degree of confidence (k = 1). These findings demonstrate the potential use of this recombinant protein in immune diagnosis of canine leishmaniasis and open the possibility of its application to other diagnostic approaches, such as immunochromatography fast lateral flow assays and human leishmaniasis diagnosis.2015-04-01T17:36:03Z2015-04-01T17:36:03Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, R. F. de et al. Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis. Acta Tropica, v. 125, p. 60-66, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X12003166#>. Acesso em: 08 nov. 2014.0001-706Xhttp://www.repositorio.ufop.br/handle/123456789/4928https://doi.org/10.1016/j.actatropica.2012.09.011ark:/61566/0013000003b0hO periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3544871302050.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPSouza, Ronny Francisco deSantos, Yaro Luciolo dosVasconcellos, Raphael de SouzaPereira, Lucas BorgesCaldas, Ivo SantanaAlmeida, Márcia Rogéria deBahia, Maria TerezinhaFietto, Juliana Lopes Rangel2024-11-10T15:07:04Zoai:repositorio.ufop.br:123456789/4928Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332024-11-10T15:07:04Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
title	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
spellingShingle	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis. Souza, Ronny Francisco de Canine leishmaniasis Immunodiagnosis Recombinant protein Ecto nucleoside triphosphate Diphosphohydrolase
title_short	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
title_full	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
title_fullStr	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
title_full_unstemmed	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
title_sort	Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.
dc.creator.none.fl_str_mv	Souza, Ronny Francisco de Santos, Yaro Luciolo dos Vasconcellos, Raphael de Souza Pereira, Lucas Borges Caldas, Ivo Santana Almeida, Márcia Rogéria de Bahia, Maria Terezinha Fietto, Juliana Lopes Rangel
author	Souza, Ronny Francisco de
author_facet	Souza, Ronny Francisco de Santos, Yaro Luciolo dos Vasconcellos, Raphael de Souza Pereira, Lucas Borges Caldas, Ivo Santana Almeida, Márcia Rogéria de Bahia, Maria Terezinha Fietto, Juliana Lopes Rangel
author_role	author
author2	Santos, Yaro Luciolo dos Vasconcellos, Raphael de Souza Pereira, Lucas Borges Caldas, Ivo Santana Almeida, Márcia Rogéria de Bahia, Maria Terezinha Fietto, Juliana Lopes Rangel
author2_role	author author author author author author author
dc.subject.por.fl_str_mv	Canine leishmaniasis Immunodiagnosis Recombinant protein Ecto nucleoside triphosphate Diphosphohydrolase
topic	Canine leishmaniasis Immunodiagnosis Recombinant protein Ecto nucleoside triphosphate Diphosphohydrolase
description	Canine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection worldwide. Leishmania (Leishmania) infantum (L. chagasi) is the etiologic agent of visceral leishmaniasis in the New World. In this work, a new L. (L.) infantum (L. chagasi) recombinant antigen, named ATP diphosphohydrolase (rLic-NTPDase-2), intended for use in the immunodiagnosis of CVL was produced and validated. The extracellular domain of ATP diphosphohydrolase was cloned and expressed in the pET21b-Escherichia coli expression system. Indirect ELISA assays were used to detect the purified rLic-NTPDase-2 antigen using a standard canine sera library. This library contained CVL-positive samples, leishmaniasis-negative samples and samples from Trypanosoma cruzi-infected dogs. The results show a high sensitivity of 100% (95% CI = 92.60–100.0%) and a high specificity of 100% (95% CI = 86.77–100.0%), with a high degree of confidence (k = 1). These findings demonstrate the potential use of this recombinant protein in immune diagnosis of canine leishmaniasis and open the possibility of its application to other diagnostic approaches, such as immunochromatography fast lateral flow assays and human leishmaniasis diagnosis.
publishDate	2013
dc.date.none.fl_str_mv	2013 2015-04-01T17:36:03Z 2015-04-01T17:36:03Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	SOUZA, R. F. de et al. Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis. Acta Tropica, v. 125, p. 60-66, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X12003166#>. Acesso em: 08 nov. 2014. 0001-706X http://www.repositorio.ufop.br/handle/123456789/4928 https://doi.org/10.1016/j.actatropica.2012.09.011
dc.identifier.dark.fl_str_mv	ark:/61566/0013000003b0h
identifier_str_mv	SOUZA, R. F. de et al. Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis. Acta Tropica, v. 125, p. 60-66, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X12003166#>. Acesso em: 08 nov. 2014. 0001-706X ark:/61566/0013000003b0h
url	http://www.repositorio.ufop.br/handle/123456789/4928 https://doi.org/10.1016/j.actatropica.2012.09.011
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP
instname_str	Universidade Federal de Ouro Preto (UFOP)
instacron_str	UFOP
institution	UFOP
reponame_str	Repositório Institucional da UFOP
collection	Repositório Institucional da UFOP
repository.name.fl_str_mv	Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv	repositorio@ufop.edu.br
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score	15,223283

Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.

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