Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)

The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different...

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Detalhes bibliográficos
Autores: Vasconcellos, R. C. C., Lima, T. F. C., Fernandes-Brum, C. N., Chalfun-Junior, A., Santos, J. B.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Recursos:Universidade Federal de Lavras (UFLA)
Repositorio:Repositório Institucional da UFLA
Idioma:inglés
OAI Identifier:oai:repositorio.ufla.br:1/29898
Acesso em linha:https://repositorio.ufla.br/handle/1/29898
Access Level:acceso abierto
Palavra-chave:Sclerotinia sclerotiorum
Polygalacturonase-inhibiting protein
Endopolygalacturonase
qPCR
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dc.title.pt_BR.fl_str_mv Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
title Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
spellingShingle Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
Vasconcellos, R. C. C.
Sclerotinia sclerotiorum
Polygalacturonase-inhibiting protein
Endopolygalacturonase
qPCR
title_short Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
title_full Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
title_fullStr Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
title_full_unstemmed Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
title_sort Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)
dc.creator.none.fl_str_mv Vasconcellos, R. C. C.
Lima, T. F. C.
Fernandes-Brum, C. N.
Chalfun-Junior, A.
Santos, J. B.
author Vasconcellos, R. C. C.
author_facet Vasconcellos, R. C. C.
Lima, T. F. C.
Fernandes-Brum, C. N.
Chalfun-Junior, A.
Santos, J. B.
author_role author
author2 Lima, T. F. C.
Fernandes-Brum, C. N.
Chalfun-Junior, A.
Santos, J. B.
author2_role author
author
author
author
dc.subject.por.fl_str_mv Sclerotinia sclerotiorum
Polygalacturonase-inhibiting protein
Endopolygalacturonase
qPCR
topic Sclerotinia sclerotiorum
Polygalacturonase-inhibiting protein
Endopolygalacturonase
qPCR
description The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different common bean (Phaseolus vulgaris L.) genotypes and its relationship with resistance to white mold (Sclerotinia sclerotiorum); ii) determine the expression levels of PvPGIP genes at different time points after inoculation with white mold; and iii) investigate differences in PvPGIP gene expression between two white mold isolates with different levels of aggressiveness. Four bean lines were analyzed, including two lines from a recurrent selection for white mold (50/5 and 84/6), one resistant line that was not adapted to Brazilian conditions (Cornell 605), and one susceptible line (Corujinha). Gene expression was investigated at 0, 1, 2, 3, and 5 days after inoculation. The isolate UFLA 03 caused no significant difference in the relative expression of any gene examined, and was inefficient in discriminating among the genotypes. For the isolate UFLA 116, all of the genes were differentially expressed, as they were associated with resistance to white mold, and the expressions increased until the third day after inoculation. The 50/5 line was not significantly different from the Corujinha line for all of the genes analyzed. However, this line had a resistance level that was similar to that of Cornell 605, according to the straw test. Therefore, the incorporation of PvPGIP genes can increase the resistance of lines derived from recurrent selection.
publishDate 2016
dc.date.issued.fl_str_mv 2016-08-18
dc.date.accessioned.fl_str_mv 2018-08-03T18:57:56Z
dc.date.available.fl_str_mv 2018-08-03T18:57:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv VASCONCELLOS, R. C. C. et al. Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.). Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, p. 1-11, Aug. 2016.
dc.identifier.uri.fl_str_mv https://repositorio.ufla.br/handle/1/29898
identifier_str_mv VASCONCELLOS, R. C. C. et al. Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.). Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, p. 1-11, Aug. 2016.
url https://repositorio.ufla.br/handle/1/29898
dc.language.iso.fl_str_mv eng
language eng
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acesso aberto
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info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-ShareAlike 4.0 International
acesso aberto
http://creativecommons.org/licenses/by-sa/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)
publisher.none.fl_str_mv Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)
dc.source.pt_BR.fl_str_mv Genetics and Molecular Research (GMR)
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institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
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spelling 2018-08-03T18:57:56Z2018-08-03T18:57:56Z2016-08-18VASCONCELLOS, R. C. C. et al. Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.). Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, p. 1-11, Aug. 2016.https://repositorio.ufla.br/handle/1/29898The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different common bean (Phaseolus vulgaris L.) genotypes and its relationship with resistance to white mold (Sclerotinia sclerotiorum); ii) determine the expression levels of PvPGIP genes at different time points after inoculation with white mold; and iii) investigate differences in PvPGIP gene expression between two white mold isolates with different levels of aggressiveness. Four bean lines were analyzed, including two lines from a recurrent selection for white mold (50/5 and 84/6), one resistant line that was not adapted to Brazilian conditions (Cornell 605), and one susceptible line (Corujinha). Gene expression was investigated at 0, 1, 2, 3, and 5 days after inoculation. The isolate UFLA 03 caused no significant difference in the relative expression of any gene examined, and was inefficient in discriminating among the genotypes. For the isolate UFLA 116, all of the genes were differentially expressed, as they were associated with resistance to white mold, and the expressions increased until the third day after inoculation. The 50/5 line was not significantly different from the Corujinha line for all of the genes analyzed. However, this line had a resistance level that was similar to that of Cornell 605, according to the straw test. Therefore, the incorporation of PvPGIP genes can increase the resistance of lines derived from recurrent selection.Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)Attribution-ShareAlike 4.0 Internationalacesso abertohttp://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessGenetics and Molecular Research (GMR)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASclerotinia sclerotiorumPolygalacturonase-inhibiting proteinEndopolygalacturonaseqPCRExpression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengVasconcellos, R. C. C.Lima, T. F. C.Fernandes-Brum, C. N.Chalfun-Junior, A.Santos, J. 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