Methods for detecting the Roundup Ready® soy in soybeans and soy products by polymerase chain reaction: a review and critical analysis of current practices of validation
Information on the origin of transgenic foods is quite relevant. According to the Brazilian and other countries legislations, the consumer must be informed about the nature of transgenic foods and ingredients, which contain or being produced from genetically modified organisms (GMOs), in amount abov...
| Autores: | , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Brasil |
| Recursos: | Instituto Adolfo Lutz |
| Repositorio: | Revista do Instituto Adolfo Lutz (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/33356 |
| Acesso em linha: | https://periodicos.saude.sp.gov.br/RIAL/article/view/33356 |
| Access Level: | acceso abierto |
| Palavra-chave: | validação de métodos reação em cadeia da polimerase organismos geneticamente modificados soja Roundup Ready® |
| Resumo: | Information on the origin of transgenic foods is quite relevant. According to the Brazilian and other countries legislations, the consumer must be informed about the nature of transgenic foods and ingredients, which contain or being produced from genetically modified organisms (GMOs), in amount above the established limit. Thus, there is a growing need for evaluating the occurrence of GMOs, and to determine the percentage of them in food. This need has generated a demand for developing methods to detect, identify and quantify the exogenous DNA. However, these methods need to be validated to ensure reliability of the results. This review paper evaluated the validation of methodologies for detecting the Roundup Ready® soybean in grain and soybean products by polymerase chain reaction. Considering the current trends in method validation, the specific guides for validating GMOs do not include all of the parameters required to assess the fitness for its purpose, especially in the case of qualitative methods. The most frequent parameters reported in the literature were accuracy, precision (repeatability), linearity and sensitivity for quantitative methods, and rates of sensitivity and selectivity for qualitative methods. However, important parameters have been neglected in the validation procedures of this analytical scope. |
|---|