Study of Potential anti-Leishmania and anti-Trypanosoma cruzi of Ergosterol isolated from Pleurotus salmoneostramineus
Considering the need for new treatments for neglected diseases asvisceral leishmaniasis and Chagas disease, in this work we fractionatedthe edible mushroom Pleurotus salmoneostramineus in the search forpotential antiparasitic compounds. Among the active fractions, it wasisolated the ergosterol, whic...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Secretaria de Estado da Saúde de São Paulo (SES-SP) |
| Repositorio: | BEPA. Boletim epidemiológico paulista (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/37838 |
| Acceso en línea: | https://periodicos.saude.sp.gov.br/BEPA182/article/view/37838 |
| Access Level: | acceso abierto |
| Palabra clave: | Ergosterol. Pleurotus salmoneostramineus Trypanosoma cruzi Leishmania infantum mecanismo de ação Ergosterol Trypanosoma cruzi. Mechanism of action |
| Sumario: | Considering the need for new treatments for neglected diseases asvisceral leishmaniasis and Chagas disease, in this work we fractionatedthe edible mushroom Pleurotus salmoneostramineus in the search forpotential antiparasitic compounds. Among the active fractions, it wasisolated the ergosterol, which showed anti-Leishmania (L.) infantume anti-Trypanosoma cruzi activities. The ergosterol was active againstintracellular amastigotes of Leishmania (L.) infantum and trypomastigotesof Trypanosoma cruzi, with 50% Inhibitory Concentration (IC50) valuesof 125 μM and 129 μM, respectively. The cytotoxicity in mammaliancells resulted in an IC50 value of 619 μM. Its action mechanism inTrypanosoma cruzi trypomastigotes resulted in permeabilization of theplasma membrane, as well as depolarization of mitochondrial membranepotential, leading to parasite death. Nevertheless, there was no increase inreactive oxygen species, demonstrating that its mechanism of action doesnot involve the induction of oxidative stress in the parasite. The selectionof antiparasitic secondary metabolites present in nature can provide futureprototypes for the design of new drugs for neglected diseases. |
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