Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades
The Pirarucu (Arapaima gigas) is one of the world's largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air-breather found in the basin of the Amazon River with an attractive potenti...
| Autores: | , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2018 |
| País: | Brasil |
| Recursos: | Universidade Federal do Rio Grande do Norte (UFRN) |
| Repositório: | Repositório Institucional da UFRN |
| Idioma: | inglês |
| OAI Identifier: | oai:repositorio.ufrn.br:123456789/25587 |
| Acesso em linha: | https://repositorio.ufrn.br/jspui/handle/123456789/25587 https://doi.org/10.1093/gbe/evy130 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Genome sequencing Phylogenomics |
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Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost cladesArapaima gigasPirarucuOsteoglossomorphaTeleosteiGenome sequencingPhylogenomicsThe Pirarucu (Arapaima gigas) is one of the world's largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air-breather found in the basin of the Amazon River with an attractive potential for aquaculture. Its phylogenetic position among bony fishes makes the Pirarucu a relevant subject for evolutionary studies of early teleost diversification. Here, we present, for the first time, a draft genome version of the A. gigas genome, providing useful information for further functional and evolutionary studies. The A. gigas genome was assembled with 103 Gb raw reads sequenced in an Illumina platform. The final draft genome assembly was approximately 661 Mb, with a contig N50 equal to 51.23 kb and scaffold N50 of 668 kb. Repeat sequences accounted for 21.69% of the whole genome, and a total of 24,655 protein-coding genes were predicted from the genome assembly, with an average of 9 exons per gene. Phylogenomic analysis based on 24 fish species supported the postulation that Osteoglossomorpha and Elopomorpha (eels, tarpons and bonefishes) are sister groups, both forming a sister lineage with respect to Clupeocephala (remaining teleosts). Divergence time estimations suggested that Osteoglossomorpha and Elopomorpha lineages emerged independently in a period of approximately 30 million years in the Jurassic. The draft genome of A. gigas provides a valuable genetic resource for further investigations of evolutionary studies and may also offer a valuable data for economic applications.2018-07-11T18:08:52Z2018-07-11T18:08:52Z2018-07-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVIALLE, R. A. et al. Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades, Genome Biology and Evolution, evy130, jul. 2018. https://doi.org/10.1093/gbe/evy130https://repositorio.ufrn.br/jspui/handle/123456789/25587https://doi.org/10.1093/gbe/evy130ark:/41046/001300000tzwxenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNVialle, Ricardo AssunçãoSouza, Jorge Estefano Santana deLopes, Katia de PaivaTeixeira, Diego GomesAlves Sobrinho, Pitágoras de AzevedoRibeiro-dos-Santos, André M.Furtado, CarolinaSakamoto, TetsuSilva, Fábio Augusto OliveiraOliveira, Edivaldo Herculano Corrêa deHamoy, Igor GuerreiroAssumpção, Paulo PimentelRibeiro-dos-Santos, ÂndreaLima, João Paulo Matos SantosSeuánez, Héctor N.Souza, Sandro José deSantos, Sidney2025-05-29T17:19:47Zoai:repositorio.ufrn.br:123456789/25587Repositório InstitucionalPUBhttp://repositorio.ufrn.br/oai/repositorio@bczm.ufrn.bropendoar:2025-05-29T17:19:47Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
| dc.title.none.fl_str_mv |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| title |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| spellingShingle |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades Vialle, Ricardo Assunção Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Genome sequencing Phylogenomics |
| title_short |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| title_full |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| title_fullStr |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| title_full_unstemmed |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| title_sort |
Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades |
| dc.creator.none.fl_str_mv |
Vialle, Ricardo Assunção Souza, Jorge Estefano Santana de Lopes, Katia de Paiva Teixeira, Diego Gomes Alves Sobrinho, Pitágoras de Azevedo Ribeiro-dos-Santos, André M. Furtado, Carolina Sakamoto, Tetsu Silva, Fábio Augusto Oliveira Oliveira, Edivaldo Herculano Corrêa de Hamoy, Igor Guerreiro Assumpção, Paulo Pimentel Ribeiro-dos-Santos, Ândrea Lima, João Paulo Matos Santos Seuánez, Héctor N. Souza, Sandro José de Santos, Sidney |
| author |
Vialle, Ricardo Assunção |
| author_facet |
Vialle, Ricardo Assunção Souza, Jorge Estefano Santana de Lopes, Katia de Paiva Teixeira, Diego Gomes Alves Sobrinho, Pitágoras de Azevedo Ribeiro-dos-Santos, André M. Furtado, Carolina Sakamoto, Tetsu Silva, Fábio Augusto Oliveira Oliveira, Edivaldo Herculano Corrêa de Hamoy, Igor Guerreiro Assumpção, Paulo Pimentel Ribeiro-dos-Santos, Ândrea Lima, João Paulo Matos Santos Seuánez, Héctor N. Souza, Sandro José de Santos, Sidney |
| author_role |
author |
| author2 |
Souza, Jorge Estefano Santana de Lopes, Katia de Paiva Teixeira, Diego Gomes Alves Sobrinho, Pitágoras de Azevedo Ribeiro-dos-Santos, André M. Furtado, Carolina Sakamoto, Tetsu Silva, Fábio Augusto Oliveira Oliveira, Edivaldo Herculano Corrêa de Hamoy, Igor Guerreiro Assumpção, Paulo Pimentel Ribeiro-dos-Santos, Ândrea Lima, João Paulo Matos Santos Seuánez, Héctor N. Souza, Sandro José de Santos, Sidney |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.subject.por.fl_str_mv |
Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Genome sequencing Phylogenomics |
| topic |
Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Genome sequencing Phylogenomics |
| description |
The Pirarucu (Arapaima gigas) is one of the world's largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air-breather found in the basin of the Amazon River with an attractive potential for aquaculture. Its phylogenetic position among bony fishes makes the Pirarucu a relevant subject for evolutionary studies of early teleost diversification. Here, we present, for the first time, a draft genome version of the A. gigas genome, providing useful information for further functional and evolutionary studies. The A. gigas genome was assembled with 103 Gb raw reads sequenced in an Illumina platform. The final draft genome assembly was approximately 661 Mb, with a contig N50 equal to 51.23 kb and scaffold N50 of 668 kb. Repeat sequences accounted for 21.69% of the whole genome, and a total of 24,655 protein-coding genes were predicted from the genome assembly, with an average of 9 exons per gene. Phylogenomic analysis based on 24 fish species supported the postulation that Osteoglossomorpha and Elopomorpha (eels, tarpons and bonefishes) are sister groups, both forming a sister lineage with respect to Clupeocephala (remaining teleosts). Divergence time estimations suggested that Osteoglossomorpha and Elopomorpha lineages emerged independently in a period of approximately 30 million years in the Jurassic. The draft genome of A. gigas provides a valuable genetic resource for further investigations of evolutionary studies and may also offer a valuable data for economic applications. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-07-11T18:08:52Z 2018-07-11T18:08:52Z 2018-07-05 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
VIALLE, R. A. et al. Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades, Genome Biology and Evolution, evy130, jul. 2018. https://doi.org/10.1093/gbe/evy130 https://repositorio.ufrn.br/jspui/handle/123456789/25587 https://doi.org/10.1093/gbe/evy130 |
| dc.identifier.dark.fl_str_mv |
ark:/41046/001300000tzwx |
| identifier_str_mv |
VIALLE, R. A. et al. Whole genome sequencing of the Pirarucu (Arapaima gigas) supports independent emergence of major teleost clades, Genome Biology and Evolution, evy130, jul. 2018. https://doi.org/10.1093/gbe/evy130 ark:/41046/001300000tzwx |
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https://repositorio.ufrn.br/jspui/handle/123456789/25587 https://doi.org/10.1093/gbe/evy130 |
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eng |
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eng |
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