The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Fundação Oswaldo Cruz (FIOCRUZ) |
| Repositorio: | Repositório Institucional da FIOCRUZ (ARCA) |
| Idioma: | portugués |
| OAI Identifier: | oai:arca.fiocruz.br:icict/34259 |
| Acceso en línea: | https://arca.fiocruz.br/handle/icict/34259 |
| Access Level: | acceso abierto |
| Palabra clave: | Infecção por Trypanosoma cruzi TcRad51 Rad51 Recombinase DNA Repair Trypanosoma cruzi Infection Parasitic Diseases Recombinasa Rad51 Reparación del ADN Enfermedades Parasitarias Reparo do DNA Doenças Parasitárias |
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| dc.title.none.fl_str_mv |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| title |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| spellingShingle |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions Silva, Danielle Gomes Passos Infecção por Trypanosoma cruzi TcRad51 Rad51 Recombinase DNA Repair Trypanosoma cruzi Infection Parasitic Diseases Recombinasa Rad51 Reparación del ADN Enfermedades Parasitarias Rad51 Recombinase Reparo do DNA Doenças Parasitárias |
| title_short |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| title_full |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| title_fullStr |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| title_full_unstemmed |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| title_sort |
The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions |
| dc.creator.none.fl_str_mv |
Silva, Danielle Gomes Passos Santos, Selma da Silva Nardelli, Sheila Cristina Mendes, Isabela Cecília Freire, Anna Cláudia Guimarães Repolês, Bruno Marçal Resende, Bruno Carvalho Costa-Silva, Héllida Marina Silva, Verônica Santana da Oliveira, Karla Andrade de Oliveira, Camila Franco Batista Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Franco, Glória Regina Macedo, Andréa Mara Pena, Sergio Danilo Junho Tahara, Erich Birelli Sales Junior, Policarpo Ademar Moreira, Douglas Souza Teixeira, Santuza Maria Ribeiro Mcculloch, Richard Virgilio, Stela Tosi, Luiz Ricardo Orsini Schenkman, Sergio Andrade, Luciana Oliveira Murta, Silvane Maria Fonseca Machado, Carlos Renato |
| author |
Silva, Danielle Gomes Passos |
| author_facet |
Silva, Danielle Gomes Passos Santos, Selma da Silva Nardelli, Sheila Cristina Mendes, Isabela Cecília Freire, Anna Cláudia Guimarães Repolês, Bruno Marçal Resende, Bruno Carvalho Costa-Silva, Héllida Marina Silva, Verônica Santana da Oliveira, Karla Andrade de Oliveira, Camila Franco Batista Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Franco, Glória Regina Macedo, Andréa Mara Pena, Sergio Danilo Junho Tahara, Erich Birelli Sales Junior, Policarpo Ademar Moreira, Douglas Souza Teixeira, Santuza Maria Ribeiro Mcculloch, Richard Virgilio, Stela Tosi, Luiz Ricardo Orsini Schenkman, Sergio Andrade, Luciana Oliveira Murta, Silvane Maria Fonseca Machado, Carlos Renato |
| author_role |
author |
| author2 |
Santos, Selma da Silva Nardelli, Sheila Cristina Mendes, Isabela Cecília Freire, Anna Cláudia Guimarães Repolês, Bruno Marçal Resende, Bruno Carvalho Costa-Silva, Héllida Marina Silva, Verônica Santana da Oliveira, Karla Andrade de Oliveira, Camila Franco Batista Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Franco, Glória Regina Macedo, Andréa Mara Pena, Sergio Danilo Junho Tahara, Erich Birelli Sales Junior, Policarpo Ademar Moreira, Douglas Souza Teixeira, Santuza Maria Ribeiro Mcculloch, Richard Virgilio, Stela Tosi, Luiz Ricardo Orsini Schenkman, Sergio Andrade, Luciana Oliveira Murta, Silvane Maria Fonseca Machado, Carlos Renato |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.other.none.fl_str_mv |
Infecção por Trypanosoma cruzi TcRad51 |
| topic |
Infecção por Trypanosoma cruzi TcRad51 Rad51 Recombinase DNA Repair Trypanosoma cruzi Infection Parasitic Diseases Recombinasa Rad51 Reparación del ADN Enfermedades Parasitarias Rad51 Recombinase Reparo do DNA Doenças Parasitárias |
| dc.subject.en.none.fl_str_mv |
Rad51 Recombinase DNA Repair Trypanosoma cruzi Infection Parasitic Diseases |
| dc.subject.es.none.fl_str_mv |
Recombinasa Rad51 Reparación del ADN Enfermedades Parasitarias |
| dc.subject.decs.none.fl_str_mv |
Rad51 Recombinase Reparo do DNA Doenças Parasitárias |
| description |
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. |
| publishDate |
2018 |
| dc.date.issued.fl_str_mv |
2018 |
| dc.date.accessioned.fl_str_mv |
2019-07-18T17:03:59Z |
| dc.date.available.fl_str_mv |
2019-07-18T17:03:59Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
SILVA, Danielle Gomes Passos et al. The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions. PLoS Neglected Tropical Diseases, v. 12, n. 11, p. 1–25, 2018. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/34259 |
| dc.identifier.issn.none.fl_str_mv |
1935-2735 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0006875 |
| dc.identifier.eissn.none.fl_str_mv |
1935-2735 |
| identifier_str_mv |
SILVA, Danielle Gomes Passos et al. The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions. PLoS Neglected Tropical Diseases, v. 12, n. 11, p. 1–25, 2018. 1935-2735 10.1371/journal.pntd.0006875 |
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https://arca.fiocruz.br/handle/icict/34259 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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2019-07-18T17:03:59Z2019-07-18T17:03:59Z2018SILVA, Danielle Gomes Passos et al. The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions. PLoS Neglected Tropical Diseases, v. 12, n. 11, p. 1–25, 2018.1935-2735https://arca.fiocruz.br/handle/icict/3425910.1371/journal.pntd.00068751935-2735porPublic Library of ScienceInfecção por Trypanosoma cruziTcRad51Rad51 RecombinaseDNA RepairTrypanosoma cruzi InfectionParasitic DiseasesRecombinasa Rad51Reparación del ADNEnfermedades ParasitariasRad51 RecombinaseReparo do DNADoenças ParasitáriasThe in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Wellcome Trust Centre for Molecular Parasitology. Glasgow Biomedical Research Centre. University of Glasgow. Glasgow, Scotland, United Kingdom.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular. Ribeirão Preto, SP, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal de Minas Gerais. Departamento de Morfologia. ICB. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.In Trypanosoma cruzi, the etiologic agent of Chagas disease, Rad51 (TcRad51) is a central enzyme for homologous recombination. Here we describe the different roles of TcRad51 in DNA repair. Epimastigotes of T. cruzi overexpressing TcRAD51 presented abundant TcRad51-labeled foci before gamma irradiation treatment, and a faster growth recovery when compared to single-knockout epimastigotes for RAD51. Overexpression of RAD51 also promoted increased resistance against hydrogen peroxide treatment, while the single-knockout epimastigotes for RAD51 exhibited increased sensitivity to this oxidant agent, which indicates a role for this gene in the repair of DNA oxidative lesions. In contrast, TcRad51 was not involved in the repair of crosslink lesions promoted by UV light and cisplatin treatment. Also, RAD51 single-knockout epimastigotes showed a similar growth rate to that exhibited by wild-type ones after treatment with hydroxyurea, but an increased sensitivity to methyl methane sulfonate. Besides its role in epimastigotes, TcRad51 is also important during mammalian infection, as shown by increased detection of T. cruzi cells overexpressing RAD51, and decreased detection of single-knockout cells for RAD51, in both fibroblasts and macrophages infected with amastigotes. Besides that, RAD51-overexpressing parasites infecting mice also presented increased infectivity and higher resistance against benznidazole. We thus show that TcRad51 is involved in the repair of DNA double strands breaks and oxidative lesions in two different T. cruzi developmental stages, possibly playing an important role in the infectivity of this parasite.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZSilva, Danielle Gomes PassosSantos, Selma da SilvaNardelli, Sheila CristinaMendes, Isabela CecíliaFreire, Anna Cláudia GuimarãesRepolês, Bruno MarçalResende, Bruno CarvalhoCosta-Silva, Héllida MarinaSilva, Verônica Santana daOliveira, Karla Andrade deOliveira, Camila Franco BatistaVilela, Liza Figueiredo FelicoriNagem, Ronaldo Alves PintoFranco, Glória ReginaMacedo, Andréa MaraPena, Sergio Danilo JunhoTahara, Erich BirelliSales Junior, Policarpo AdemarMoreira, Douglas SouzaTeixeira, Santuza Maria RibeiroMcculloch, RichardVirgilio, StelaTosi, Luiz Ricardo OrsiniSchenkman, SergioAndrade, Luciana OliveiraMurta, Silvane Maria FonsecaMachado, Carlos RenatoLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://arca.fiocruz.br/bitstreams/13161e75-6b20-436e-aa61-5f807cd8b776/download783568c2893d2e25a99990b126be1772MD51falseAnonymousREADORIGINALjournal.pntd.000682.pdfjournal.pntd.000682.pdfapplication/pdf2978415https://arca.fiocruz.br/bitstreams/71cb079f-366d-4406-bd12-d27dd61984a8/download8f8f5e8a8c402a668b4846dbfc7b9afcMD52trueAnonymousREADTEXTjournal.pntd.000682.pdf.txtjournal.pntd.000682.pdf.txtExtracted texttext/plain95253https://arca.fiocruz.br/bitstreams/bcdb64c7-5d9e-4a09-b276-b48daea0e35d/download99b35b15f857c3f5e8a51c97167aa33dMD57falseAnonymousREADTHUMBNAILjournal.pntd.000682.pdf.jpgjournal.pntd.000682.pdf.jpgGenerated Thumbnailimage/jpeg48314https://arca.fiocruz.br/bitstreams/a228142d-d1b9-4b73-97ee-c0474c38a2f3/download741c47729d7334120c6633a6411dfb39MD58falseAnonymousREADicict/342592025-12-09 15:15:30.777open.accessoai:arca.fiocruz.br:icict/34259https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-09T18:15:30Repositório Institucional da FIOCRUZ (ARCA) - 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| score |
15,301629 |