Toxicity Testing of Restorative Dental Materials Using Brine Shrimp Larvae (Artemia salina)

This study investigated the effect of extracts of different composites, glass ionomer cement (GIC)s and compomers on the viability of brine shrimp larvae. Ethanolic extracts of four dental composites (Z-100; Solitaire 2; Filtek P60 and Synergy), a conventional GIC (Ketac-Fil), a resin-modified glass...

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Bibliographic Details
Authors: Milhem, Manar M., Al-Hiyasat, Ahmad S., Darmani, Homa
Format: article
Status:Published version
Publication Date:2008
Country:Brasil
Institution:Universidade de São Paulo (USP)
Repository:Journal of applied oral science (Online)
Language:English
OAI Identifier:oai:revistas.usp.br:article/3585
Online Access:https://www.revistas.usp.br/jaos/article/view/3585
Access Level:Open access
Keyword:Glass ionomer cements
Compomers
Artemia salina
Description
Summary:This study investigated the effect of extracts of different composites, glass ionomer cement (GIC)s and compomers on the viability of brine shrimp larvae. Ethanolic extracts of four dental composites (Z-100; Solitaire 2; Filtek P60 and Synergy), a conventional GIC (Ketac-Fil), a resin-modified glass ionomer cement (Vitremer), two compomers (F2000; Dyract AP), and a flowable compomer (Dyract Flow) were prepared from each material. Following evaporation of the ethanol, the extracts were resuspended in distilled water, which was then used to test the effects on the viability of brine shrimp larvae. For the composites, the extract of Synergy was the least toxic (88% viability) followed by the extracts of Solitaire 2, Z100 and P60 (75%, 67.5% and 50% viability, respectively). One-way ANOVA revealed highly significant differences between the resin composite materials (p