Breeding protocol for the sand fly Nyssomyia neivai (Diptera: Psychodidae) in laboratory conditions

The information in this protocol covers from the basic steps and material necessary to start a sand fly colony up to the specific details which are important to the success of a Nyssomyia neivai colony. The greatest problems in our colony of Ny. neivai were solved with specific care, for instance, u...

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Detalles Bibliográficos
Autores: Goulart, Thais Marchi, da Rocha Silva, Flávia Benini [UNESP], Machado, Vicente Estevam [UNESP], Oliveira, Wanderson Henrique Cruz [UNESP], de Castro, Camila Feitosa [UNESP], Rodrigues, Marili Villa Nova, Pinto, Mara Cristina [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/179024
Acceso en línea:http://dx.doi.org/10.1016/j.actatropica.2017.07.010
http://hdl.handle.net/11449/179024
Access Level:acceso abierto
Palabra clave:Colonization
Colony
Life cycle
Phlebotomine
Rearing methods
Sand flies
Descripción
Sumario:The information in this protocol covers from the basic steps and material necessary to start a sand fly colony up to the specific details which are important to the success of a Nyssomyia neivai colony. The greatest problems in our colony of Ny. neivai were solved with specific care, for instance, using vermiculite and an adequate number of adults in oviposition containers; the control of fungus with the exact amount of diet for the larvae and humidity control; a second blood meal for females and control of the number of times animals are used for blood meals. Currently, our colony is at F22 generation.