Comparison of five methods of extraction of staphylococcus aureus dna for molecular detection by pcr
Introduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results:...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2018 |
| Country: | Brasil |
| Institution: | Universidade Federal de Minas Gerais (UFMG) |
| Repository: | Repositório Institucional da UFMG |
| Language: | English |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/63266 |
| Online Access: | http://hdl.handle.net/1843/63266 |
| Access Level: | Open access |
| Keyword: | Polymerase Chain Reaction DNA, Bacterial DNA extraction Nosocomial infection Cross Infection |
| Summary: | Introduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols |
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