Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Recife, PE, Brasil
| Autores: | , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Institución: | Fundação Oswaldo Cruz (FIOCRUZ) |
| Repositorio: | Repositório Institucional da FIOCRUZ (ARCA) |
| Idioma: | inglés |
| OAI Identifier: | oai:arca.fiocruz.br:icict/19544 |
| Acceso en línea: | https://arca.fiocruz.br/handle/icict/19544 |
| Access Level: | acceso abierto |
| Palabra clave: | Cutaneous leishmaniasis diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis Leishmaniose Cutânea Proteínas Recombinantes |
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| dc.title.none.fl_str_mv |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| spellingShingle |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species Sato, Camila Massae Cutaneous leishmaniasis diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis Leishmaniose Cutânea Proteínas Recombinantes |
| title_short |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_full |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_fullStr |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_full_unstemmed |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_sort |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| dc.creator.none.fl_str_mv |
Sato, Camila Massae Sanchez, Maria Carmen Arroyo Celeste, Beatriz Julieta Duthie, Malcolm S. Guderian, Jeffrey Reed, Steven G. Brito, Maria Edileuza Felinto de Campos, Marliane Batista de Souza Encarnação, Helia Valeria Guerra, Jorge de Mesquita, Tirza Gabrielle Ramos Pinheiro, Suzana Kanawati Ramasawmy, Rajendranath Silveira, Fernando Tobias de Assis Souza, Marina Goto, Hiro |
| author |
Sato, Camila Massae |
| author_facet |
Sato, Camila Massae Sanchez, Maria Carmen Arroyo Celeste, Beatriz Julieta Duthie, Malcolm S. Guderian, Jeffrey Reed, Steven G. Brito, Maria Edileuza Felinto de Campos, Marliane Batista de Souza Encarnação, Helia Valeria Guerra, Jorge de Mesquita, Tirza Gabrielle Ramos Pinheiro, Suzana Kanawati Ramasawmy, Rajendranath Silveira, Fernando Tobias de Assis Souza, Marina Goto, Hiro |
| author_role |
author |
| author2 |
Sanchez, Maria Carmen Arroyo Celeste, Beatriz Julieta Duthie, Malcolm S. Guderian, Jeffrey Reed, Steven G. Brito, Maria Edileuza Felinto de Campos, Marliane Batista de Souza Encarnação, Helia Valeria Guerra, Jorge de Mesquita, Tirza Gabrielle Ramos Pinheiro, Suzana Kanawati Ramasawmy, Rajendranath Silveira, Fernando Tobias de Assis Souza, Marina Goto, Hiro |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.subject.en.none.fl_str_mv |
Cutaneous leishmaniasis diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis |
| topic |
Cutaneous leishmaniasis diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis Leishmaniose Cutânea Proteínas Recombinantes |
| dc.subject.decs.none.fl_str_mv |
Leishmaniose Cutânea Proteínas Recombinantes |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Recife, PE, Brasil |
| publishDate |
2017 |
| dc.date.accessioned.fl_str_mv |
2017-06-27T19:33:55Z |
| dc.date.available.fl_str_mv |
2017-06-27T19:33:55Z |
| dc.date.issued.fl_str_mv |
2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SATO, C. M. et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495–503, fev. 2017. DOI 10.1128/JCM.01904-16. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/19544 |
| dc.identifier.issn.none.fl_str_mv |
1098-660X |
| dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.01904-16 |
| identifier_str_mv |
SATO, C. M. et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495–503, fev. 2017. DOI 10.1128/JCM.01904-16. 1098-660X 10.1128/JCM.01904-16 |
| url |
https://arca.fiocruz.br/handle/icict/19544 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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2017-06-27T19:33:55Z2017-06-27T19:33:55Z2017SATO, C. M. et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495–503, fev. 2017. DOI 10.1128/JCM.01904-16.1098-660Xhttps://arca.fiocruz.br/handle/icict/1954410.1128/JCM.01904-16engUse of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães, Recife, PE, BrasilAmerican tegumentary leishmaniasis (ATL) (also known as cutaneous leishmaniasis [CL]) is caused by various species of protozoa of the genus Leishmania The diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular Leishmania species causing the disease can lead to variable performance. In the present study, we generated recombinant versions of two highly conserved Leishmania proteins, Leishmania (Viannia) braziliensis-derived Lb8E and Lb6H, and evaluated both in enzyme-linked immunosorbent assays (ELISA). Recombinant Lb6H (rLb6H) had better performance and reacted with 100.0% of the ATL and 89.4% of the VL samples. These reactions with rLb6H were highly specific (98.5%) when compared against those for samples from healthy control individuals. We then assessed rLb6H against sera from ATL patients infected with different species of Leishmania prevalent in Brazil [Leishmania (Leishmania) amazonensis, L (Viannia) braziliensis, and L (V) guyanensis] and samples from patients with other infectious diseases. In analyses of 500 sera, ELISA using rLb6H detected all 219 ATL samples (sensitivity of 100.0%) with an overall specificity of 93.9% (considering healthy individuals and other infectious diseases patients). Only a minority of samples from Chagas disease patients possessed antibodies against rLb6H, and all of these responses were low (with a highest reactivity index of 2.2). Taken together, our data support further evaluation of rLb6H and the potential for its routine use in the serological diagnosis of ATL.Cutaneous leishmaniasisdiagnosticsELISAimmunoserologyrecombinant antigentegumentary leishmaniasisLeishmaniose CutâneaProteínas Recombinantesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZSato, Camila MassaeSanchez, Maria Carmen ArroyoCeleste, Beatriz JulietaDuthie, Malcolm S.Guderian, JeffreyReed, Steven G.Brito, Maria Edileuza Felinto deCampos, Marliane Batistade Souza Encarnação, Helia ValeriaGuerra, Jorgede Mesquita, Tirza Gabrielle RamosPinheiro, Suzana KanawatiRamasawmy, RajendranathSilveira, Fernando Tobiasde Assis Souza, MarinaGoto, HiroLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/3676b1a2-1b88-4e80-9c65-2f47a9aea572/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINAL27927927 2017 sat-use.oa.pdf27927927 2017 sat-use.oa.pdfapplication/pdf548059https://arca.fiocruz.br/bitstreams/348eb3c3-9149-4656-b3c1-8b2636b61389/download203532deb5164f6376996aeeadd65c0fMD52trueAnonymousREADTEXT27927927 2017 sat-use.oa.pdf.txt27927927 2017 sat-use.oa.pdf.txtExtracted texttext/plain42547https://arca.fiocruz.br/bitstreams/c17994ee-fe8c-4fa4-8167-598cf4e0f53f/download62819839ea4795347b066941c6d1a392MD511falseAnonymousREADTHUMBNAIL27927927 2017 sat-use.oa.pdf.jpg27927927 2017 sat-use.oa.pdf.jpgGenerated Thumbnailimage/jpeg39064https://arca.fiocruz.br/bitstreams/803c9199-0e87-4f6c-a5f8-458f64a7d1de/downloadfec2b0c5b40ce459c59563630e20b3a9MD512falseAnonymousREADicict/195442025-12-09 15:16:06.602open.accessoai:arca.fiocruz.br:icict/19544https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-09T18:16:06Repositório Institucional da FIOCRUZ (ARCA) - 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