Genetic diversity in natural populations of Pfaffia glomerata (Spreng.) Pedersen estimated by RAPD markers - DOI: 10.4025/actasciagron.v31i3.548

Pfaffia glomerata is a native species from South America, occurring naturally in some states of Brazil. The ever growing interest by the species, mainly due to its important therapeutic properties, has threatened the natural populations, as exploitation has occurred in a rather predatory way. The pr...

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Detalhes bibliográficos
Autores: Kamada, Takeshi, Picoli, Edgard Augusto de Toledo, Alfenas, Acelino Couto, Cruz, Cosme Damião, Vieira, Roberto Fontes, Otoni, Wagner Campos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Brasil
Recursos:Universidade Estadual de Maringá (UEM)
Repositorio:Acta Scientiarum. Agronomy (Online)
Idioma:portugués
OAI Identifier:oai:periodicos.uem.br/ojs:article/548
Acesso em linha:http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/548
Access Level:acceso abierto
Palavra-chave:brazilian-ginseng
germplasm conservation
plant breeding
‘ginseng brasileiro’
conservação de germoplasma
melhoramento vegetal
5.01.03.05-9
Descrição
Resumo:Pfaffia glomerata is a native species from South America, occurring naturally in some states of Brazil. The ever growing interest by the species, mainly due to its important therapeutic properties, has threatened the natural populations, as exploitation has occurred in a rather predatory way. The present study aimed to compare the genetic diversity of four natural populations originated from of the area of the Paraná River basin (Paraná and Mato Grosso States). The individuals were propagated in vitro and characterized by means of RAPD markers. A total of 267 polymorphic marks were obtained from 67 primers. The genetic distance estimates were obtained based on the complement arithmetic of Nei and Li’s similarity coefficient, and the genotypes were grouped according to the UPGMA method. Three populations from the margin of the Paraná River and at Mineiro Island presented low diversity levels among them, though possessing the same propagule dispersal flow. The population located in the proximities of the margin of the Ivaí river (tributary of the river Paraná) presented greater diversity values within and among the populations, suggesting that its preservation should be a priority and that the origin of the genetic variability may be related to the dispersion flow of the vegetative propagules.