Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis

Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family mem- bers, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which...

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Autores: Frasson, Amanda Piccoli, Charão, Mariele Feiffer, Rosemberg, Denis Broock, Souza, Ana Paula de, Garcia, Solange Cristina, Bonorino, Cristina Beatriz Cazabuena, Bogo, Mauricio Reis, De Carli, Geraldo Attilio, Tasca, Tiana
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:Brasil
Recursos:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositório:Repositório Institucional da UFRGS
Idioma:inglês
OAI Identifier:oai:www.lume.ufrgs.br:10183/72280
Acesso em linha:http://hdl.handle.net/10183/72280
Access Level:Acceso aberto
Palavra-chave:Trichomonas vaginalis
Ecto-5'-nucleotidase
Adenosina
Serum limitation
Ectonucleoside triphosphate diphosphohydrolase
Ecto-5’
Nucleotidase
Adenosine
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spelling 2013-06-07T01:45:24Z20120074-0276http://hdl.handle.net/10183/72280000873516Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family mem- bers, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which hydrolyses AMP, have been char- acterised in T. vaginalis. For trichomonad culture, the growth medium is supplemented with 10% serum, which is an important source of nutrients, such as adenosine. Here, we investigated the ATP metabolism of T. vaginalis tropho- zoites from long-term cultures and clinical isolates under limited bovine serum conditions (1% serum). The specific enzymatic activities were expressed as nmol inorganic phosphate (Pi) released/min/mg protein, the gene expression patterns were determined by reverse transcriptase-polymerase chain reaction, the extracellular adenine nucleotide hydrolysis was analysed by high performance liquid chromatography and the cell cycle analysis was assessed by f low cytometry. Serum limitation led to the profound activation of NTPDase and ecto-5’-nucleotidase activities. Furthermore, the levels of NTPDase A and B transcripts increased and extracellular ATP metabolism was activated, which led to enhanced ATP hydrolysis and the formation of ADP and AMP. Moreover, the cell cycle was arrested at the G0/G1 stage, which suggested adenosine uptake. Our data suggest that under conditions of serum limitation, NTPDase and ecto-5’-nucleotidase play a role in providing the adenosine required for T. vaginalis growth and that this process contributes to the establishment of parasitism.application/pdfengMemórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 107, n. 2 (mar. 2012), p. 170-177Trichomonas vaginalisEcto-5'-nucleotidaseAdenosinaTrichomonas vaginalisSerum limitationEctonucleoside triphosphate diphosphohydrolaseEcto-5’NucleotidaseAdenosineAnalysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalisinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSFrasson, Amanda PiccoliCharão, Mariele FeifferRosemberg, Denis BroockSouza, Ana Paula deGarcia, Solange CristinaBonorino, Cristina Beatriz CazabuenaBogo, Mauricio ReisDe Carli, Geraldo AttilioTasca, TianaORIGINAL000873516.pdf000873516.pdfTexto completo (inglês)application/pdf659921http://www.lume.ufrgs.br/bitstream/10183/72280/1/000873516.pdf05a7475c80b14c7e27a69de63d522109MD51TEXT000873516.pdf.txt000873516.pdf.txtExtracted Texttext/plain41307http://www.lume.ufrgs.br/bitstream/10183/72280/2/000873516.pdf.txteb0844a1ed72368139a26a776bfa9376MD52THUMBNAIL000873516.pdf.jpg000873516.pdf.jpgGenerated Thumbnailimage/jpeg2101http://www.lume.ufrgs.br/bitstream/10183/72280/3/000873516.pdf.jpg3758e6f74955ad78cedc65684547778bMD5310183/722802018-10-15 09:04:21.717oai:www.lume.ufrgs.br:10183/72280Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2018-10-15T12:04:21Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
title Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
spellingShingle Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
Frasson, Amanda Piccoli
Trichomonas vaginalis
Ecto-5'-nucleotidase
Adenosina
Trichomonas vaginalis
Serum limitation
Ectonucleoside triphosphate diphosphohydrolase
Ecto-5’
Nucleotidase
Adenosine
title_short Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
title_full Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
title_fullStr Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
title_full_unstemmed Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
title_sort Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis
dc.creator.none.fl_str_mv Frasson, Amanda Piccoli
Charão, Mariele Feiffer
Rosemberg, Denis Broock
Souza, Ana Paula de
Garcia, Solange Cristina
Bonorino, Cristina Beatriz Cazabuena
Bogo, Mauricio Reis
De Carli, Geraldo Attilio
Tasca, Tiana
author Frasson, Amanda Piccoli
author_facet Frasson, Amanda Piccoli
Charão, Mariele Feiffer
Rosemberg, Denis Broock
Souza, Ana Paula de
Garcia, Solange Cristina
Bonorino, Cristina Beatriz Cazabuena
Bogo, Mauricio Reis
De Carli, Geraldo Attilio
Tasca, Tiana
author_role author
author2 Charão, Mariele Feiffer
Rosemberg, Denis Broock
Souza, Ana Paula de
Garcia, Solange Cristina
Bonorino, Cristina Beatriz Cazabuena
Bogo, Mauricio Reis
De Carli, Geraldo Attilio
Tasca, Tiana
author2_role author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Trichomonas vaginalis
Ecto-5'-nucleotidase
Adenosina
topic Trichomonas vaginalis
Ecto-5'-nucleotidase
Adenosina
Trichomonas vaginalis
Serum limitation
Ectonucleoside triphosphate diphosphohydrolase
Ecto-5’
Nucleotidase
Adenosine
dc.subject.eng.fl_str_mv Trichomonas vaginalis
Serum limitation
Ectonucleoside triphosphate diphosphohydrolase
Ecto-5’
Nucleotidase
Adenosine
description Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family mem- bers, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which hydrolyses AMP, have been char- acterised in T. vaginalis. For trichomonad culture, the growth medium is supplemented with 10% serum, which is an important source of nutrients, such as adenosine. Here, we investigated the ATP metabolism of T. vaginalis tropho- zoites from long-term cultures and clinical isolates under limited bovine serum conditions (1% serum). The specific enzymatic activities were expressed as nmol inorganic phosphate (Pi) released/min/mg protein, the gene expression patterns were determined by reverse transcriptase-polymerase chain reaction, the extracellular adenine nucleotide hydrolysis was analysed by high performance liquid chromatography and the cell cycle analysis was assessed by f low cytometry. Serum limitation led to the profound activation of NTPDase and ecto-5’-nucleotidase activities. Furthermore, the levels of NTPDase A and B transcripts increased and extracellular ATP metabolism was activated, which led to enhanced ATP hydrolysis and the formation of ADP and AMP. Moreover, the cell cycle was arrested at the G0/G1 stage, which suggested adenosine uptake. Our data suggest that under conditions of serum limitation, NTPDase and ecto-5’-nucleotidase play a role in providing the adenosine required for T. vaginalis growth and that this process contributes to the establishment of parasitism.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-06-07T01:45:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/other
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/72280
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.nrb.pt_BR.fl_str_mv 000873516
identifier_str_mv 0074-0276
000873516
url http://hdl.handle.net/10183/72280
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Memórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 107, n. 2 (mar. 2012), p. 170-177
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instacron:UFRGS
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instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
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