Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples

The microscopic methods for detecting Cryptosporidium and Giardia are expensive, and a variable reproducibility and a low recovery are found. Nevertheless, a rigorous technical standardization and a quality control in the recovery testing give support to use them for analyzing the environmental samp...

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Autor: Stancari, Regina Célia Arantes
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Recursos:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/32923
Acesso em linha:https://periodicos.saude.sp.gov.br/RIAL/article/view/32923
Access Level:acceso abierto
Palavra-chave:Cryptosporidium spp.
Giardia spp.
ELISA
detection limits
limite de detecção
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spelling Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samplesDeterminação da sensibilidade do teste de ELISA para pesquisa de Cryptosporidium spp. e Giardia spp. em amostras de águas brutasCryptosporidium spp.Giardia spp.ELISAdetection limitsCryptosporidium spp.Giardia spp.ELISAlimite de detecçãoThe microscopic methods for detecting Cryptosporidium and Giardia are expensive, and a variable reproducibility and a low recovery are found. Nevertheless, a rigorous technical standardization and a quality control in the recovery testing give support to use them for analyzing the environmental samples. This study determined the sensitivity of ELISA for analyzing Giardia spp. and Cryptosporidium spp. in raw water samples. Also, it was investigated whether this assay could be used as a screening test for detecting Giardia cysts and Cryptosporidium oocysts in raw water samples; and to deduce the potential transmission of giardiasis and cryptosporidiosis from these specimens. The detection limits were high, being 98 cysts and 3.492 oocysts. Of the analyzed environmental samples, 20 % showed ELISA positive for Cryptosporidium spp., and none for Giardia spp.. Therefore, ELISA showed to be unsuitable to be used as screening assay, because in the environmental samples usually occur a lower numbers of oocysts and cysts than those regularly found in infected stool samples, for which these kits have been validated. Furthermore, the dilutions prepared from the stock solutions of cysts and oocysts in distilled water could have resulted in unfavorable reaction conditions, and it has affected the sensitivity of ELISA.Os métodos microscópicos para detecção de Cryptosporidium e Giardia são dispendiosos e apresentam reprodutibilidade variável e baixa recuperação, porém a rigorosa padronização da técnica e o controle de qualidade nos testes de recuperação permitem sua utilização em amostras ambientais. Neste estudo foi determinada a sensibilidade do ELISA, analisando-se o limite de detecção. Ademais, foi verificada a viabilidade de utilizá-lo como teste de triagem nos locais em que os métodos microscópicos foram aplicados para confirmar a veiculação de oocistos e cistos íntegros, e que podem representar risco de transmissão da criptosporidiose e\ou giardíase. Os limites de detecção foram altos: 98 cistos e 3.492 oocistos. Do total de amostras ambientais analisadas, 20% foram positivas para Cryptosporidium spp. e nenhuma para Giardia spp.. Concluiu-se que ELISA não apresenta sensibilidade adequada como teste de triagem para a finalidade deste estudo, pois as amostras ambientais geralmente apresentam números de cistos e oocistos abaixo daqueles observados em amostras fecais infectadas, para as quais os kits de reagente ELISA foram validados. Além disso, as diluições das soluções estoques dos cistos e oocistos em água destilada podem ter resultado em condições de reações desfavoráveis e ter influenciado na sensibilidade do ELISA.Instituto Adolfo Lutz2013-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3292310.18241/0073-98552013721569Instituto Adolfo Lutz Journal - RIAL; Vol. 72 No. 3 (2013); 234-238Revista del Instituto Adolfo Lutz - RIAL; Vol. 72 Núm. 3 (2013); 234-238Revista do Instituto Adolfo Lutz; v. 72 n. 3 (2013); 234-2381983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/32923/31754Copyright (c) 2013 Regina Célia Arantes Stancariinfo:eu-repo/semantics/openAccessStancari, Regina Célia Arantes2023-11-07T19:37:30Zoai:ojs.periodicos.saude.sp.gov.br:article/32923Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2023-11-07T19:37:30Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
Determinação da sensibilidade do teste de ELISA para pesquisa de Cryptosporidium spp. e Giardia spp. em amostras de águas brutas
title Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
spellingShingle Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
Stancari, Regina Célia Arantes
Cryptosporidium spp.
Giardia spp.
ELISA
detection limits
Cryptosporidium spp.
Giardia spp.
ELISA
limite de detecção
title_short Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
title_full Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
title_fullStr Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
title_full_unstemmed Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
title_sort Sensitivity of ELISA for detecting Cryptosporidium spp. and Giardia spp. in raw waters samples
dc.creator.none.fl_str_mv Stancari, Regina Célia Arantes
author Stancari, Regina Célia Arantes
author_facet Stancari, Regina Célia Arantes
author_role author
dc.subject.por.fl_str_mv Cryptosporidium spp.
Giardia spp.
ELISA
detection limits
Cryptosporidium spp.
Giardia spp.
ELISA
limite de detecção
topic Cryptosporidium spp.
Giardia spp.
ELISA
detection limits
Cryptosporidium spp.
Giardia spp.
ELISA
limite de detecção
description The microscopic methods for detecting Cryptosporidium and Giardia are expensive, and a variable reproducibility and a low recovery are found. Nevertheless, a rigorous technical standardization and a quality control in the recovery testing give support to use them for analyzing the environmental samples. This study determined the sensitivity of ELISA for analyzing Giardia spp. and Cryptosporidium spp. in raw water samples. Also, it was investigated whether this assay could be used as a screening test for detecting Giardia cysts and Cryptosporidium oocysts in raw water samples; and to deduce the potential transmission of giardiasis and cryptosporidiosis from these specimens. The detection limits were high, being 98 cysts and 3.492 oocysts. Of the analyzed environmental samples, 20 % showed ELISA positive for Cryptosporidium spp., and none for Giardia spp.. Therefore, ELISA showed to be unsuitable to be used as screening assay, because in the environmental samples usually occur a lower numbers of oocysts and cysts than those regularly found in infected stool samples, for which these kits have been validated. Furthermore, the dilutions prepared from the stock solutions of cysts and oocysts in distilled water could have resulted in unfavorable reaction conditions, and it has affected the sensitivity of ELISA.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/32923
10.18241/0073-98552013721569
url https://periodicos.saude.sp.gov.br/RIAL/article/view/32923
identifier_str_mv 10.18241/0073-98552013721569
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/32923/31754
dc.rights.driver.fl_str_mv Copyright (c) 2013 Regina Célia Arantes Stancari
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2013 Regina Célia Arantes Stancari
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 72 No. 3 (2013); 234-238
Revista del Instituto Adolfo Lutz - RIAL; Vol. 72 Núm. 3 (2013); 234-238
Revista do Instituto Adolfo Lutz; v. 72 n. 3 (2013); 234-238
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
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score 15.301629

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