Agar and activated charcoal influence the in vitro development of shoot and root sequoia

Meneguzzi, Aline; Camargo, Samila Silva; Navroski, Marcio Carlos; Pereira, Mariane de Oliveira; Souza, Patricia Fukushima; Rufato, Leo

Agar and activated charcoal influence the in vitro development of shoot and root sequoia

Experimental seedlings with Sequoia sempervirens (Lamb), a species known worldwide for its large size, longevity and quality of timber, are beginning in Brazil, making the availability of quality seedlings necessary. Thus, the technique of tissue culture, via micropropagation, becomes a great ally f...

ver descrição completa

Detalhes bibliográficos
Autores:	Meneguzzi, Aline, Camargo, Samila Silva, Navroski, Marcio Carlos, Pereira, Mariane de Oliveira, Souza, Patricia Fukushima, Rufato, Leo
Formato:	artículo
Estado:	Versión publicada
Fecha de publicación:	2020
País:	Brasil
Recursos:	Universidade do Estado de Santa Catarina (UDESC)
Repositorio:	Revista de Ciências Agroveterinárias (Online)
Idioma:	portugués
OAI Identifier:	oai::article/10791
Acesso em linha:	https://periodicos.udesc.br/index.php/agroveterinaria/article/view/10791
Access Level:	acceso abierto
Palavra-chave:	Sequoia sempervirens (Lamb) woody species micropropagation rooting in vitro espécie lenhosa micropropagação enraizamento in vitro

id	BR_036393a00b42b70603d340b4e589e996
oai_identifier_str	oai::article/10791
network_acronym_str	BR
network_name_str	Brasil
repository_id_str
dc.title.none.fl_str_mv	Agar and activated charcoal influence the in vitro development of shoot and root sequoia Ágar e carvão ativado influenciam no desenvolvimento in vitro da parte aérea e radicular de sequoia
title	Agar and activated charcoal influence the in vitro development of shoot and root sequoia
spellingShingle	Agar and activated charcoal influence the in vitro development of shoot and root sequoia Meneguzzi, Aline Sequoia sempervirens (Lamb) woody species micropropagation rooting in vitro Sequoia sempervirens (Lamb) espécie lenhosa micropropagação enraizamento in vitro
title_short	Agar and activated charcoal influence the in vitro development of shoot and root sequoia
title_full	Agar and activated charcoal influence the in vitro development of shoot and root sequoia
title_fullStr	Agar and activated charcoal influence the in vitro development of shoot and root sequoia
title_full_unstemmed	Agar and activated charcoal influence the in vitro development of shoot and root sequoia
title_sort	Agar and activated charcoal influence the in vitro development of shoot and root sequoia
dc.creator.none.fl_str_mv	Meneguzzi, Aline Camargo, Samila Silva Navroski, Marcio Carlos Pereira, Mariane de Oliveira Souza, Patricia Fukushima Rufato, Leo
author	Meneguzzi, Aline
author_facet	Meneguzzi, Aline Camargo, Samila Silva Navroski, Marcio Carlos Pereira, Mariane de Oliveira Souza, Patricia Fukushima Rufato, Leo
author_role	author
author2	Camargo, Samila Silva Navroski, Marcio Carlos Pereira, Mariane de Oliveira Souza, Patricia Fukushima Rufato, Leo
author2_role	author author author author author
dc.subject.por.fl_str_mv	Sequoia sempervirens (Lamb) woody species micropropagation rooting in vitro Sequoia sempervirens (Lamb) espécie lenhosa micropropagação enraizamento in vitro
topic	Sequoia sempervirens (Lamb) woody species micropropagation rooting in vitro Sequoia sempervirens (Lamb) espécie lenhosa micropropagação enraizamento in vitro
description	Experimental seedlings with Sequoia sempervirens (Lamb), a species known worldwide for its large size, longevity and quality of timber, are beginning in Brazil, making the availability of quality seedlings necessary. Thus, the technique of tissue culture, via micropropagation, becomes a great ally for the guarantee of genetic superiority, large-scale production and reduced propagation time. Currently there are numerous protocols for several species, however, it is necessary to verify all influential agents in the success of in vitro development. The objective of this work was to determine the influence of activated charcoal and agar concentrations on aerial and root development of S. sempervirens. The treatments involved the presence / absence of activated carbon (1 g L-1), combined with concentrations of agar (4, 5 and 6 g L-1) in 50% MS medium. For the number and length of shoots and roots, only activated carbon was significant, and the presence benefited the development of the explants. For the percentage of callus, there was interaction of the factors, and higher concentrations of agar (5 and 6 g L-1) in the presence of medium were harmful, since they presented higher callosity, which minimizes the rooting rates. It is indicated the addition of activated charcoal (1 g L-1) and lower concentration of agar (4 g L-1) in 50% MS culture medium for a better in vitro development of sequoia.
publishDate	2020
dc.date.none.fl_str_mv	2020-07-28
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	https://periodicos.udesc.br/index.php/agroveterinaria/article/view/10791 10.5965/2238117118e2019020
url	https://periodicos.udesc.br/index.php/agroveterinaria/article/view/10791
identifier_str_mv	10.5965/2238117118e2019020
dc.language.iso.fl_str_mv	por
language	por
dc.relation.none.fl_str_mv	https://periodicos.udesc.br/index.php/agroveterinaria/article/view/10791/pdf
dc.rights.driver.fl_str_mv	Copyright (c) 2019 Revista de Ciências Agroveterinárias (Journal of Agroveterinary Sciences) info:eu-repo/semantics/openAccess
rights_invalid_str_mv	Copyright (c) 2019 Revista de Ciências Agroveterinárias (Journal of Agroveterinary Sciences)
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidade do Estado de Santa Catarina
publisher.none.fl_str_mv	Universidade do Estado de Santa Catarina
dc.source.none.fl_str_mv	Revista de Ciências Agroveterinárias; Vol. 18 (2019): Edição Especial - XII Simpósio Florestal Catarinense; 20 - 24 Revista de Ciências Agroveterinárias; v. 18 (2019): Edição Especial - XII Simpósio Florestal Catarinense; 20 - 24 2238-1171 reponame:Revista de Ciências Agroveterinárias (Online) instname:Universidade do Estado de Santa Catarina (UDESC) instacron:UDESC
instname_str	Universidade do Estado de Santa Catarina (UDESC)
instacron_str	UDESC
institution	UDESC
reponame_str	Revista de Ciências Agroveterinárias (Online)
collection	Revista de Ciências Agroveterinárias (Online)
repository.name.fl_str_mv	Revista de Ciências Agroveterinárias (Online) - Universidade do Estado de Santa Catarina (UDESC)
repository.mail.fl_str_mv	rca.cav@udesc.br \| portal.periodicos@udesc.br
_version_	1853666103777034240
spelling	Agar and activated charcoal influence the in vitro development of shoot and root sequoiaÁgar e carvão ativado influenciam no desenvolvimento in vitro da parte aérea e radicular de sequoiaSequoia sempervirens (Lamb)woody speciesmicropropagationrooting in vitroSequoia sempervirens (Lamb)espécie lenhosamicropropagaçãoenraizamento in vitroExperimental seedlings with Sequoia sempervirens (Lamb), a species known worldwide for its large size, longevity and quality of timber, are beginning in Brazil, making the availability of quality seedlings necessary. Thus, the technique of tissue culture, via micropropagation, becomes a great ally for the guarantee of genetic superiority, large-scale production and reduced propagation time. Currently there are numerous protocols for several species, however, it is necessary to verify all influential agents in the success of in vitro development. The objective of this work was to determine the influence of activated charcoal and agar concentrations on aerial and root development of S. sempervirens. The treatments involved the presence / absence of activated carbon (1 g L-1), combined with concentrations of agar (4, 5 and 6 g L-1) in 50% MS medium. For the number and length of shoots and roots, only activated carbon was significant, and the presence benefited the development of the explants. For the percentage of callus, there was interaction of the factors, and higher concentrations of agar (5 and 6 g L-1) in the presence of medium were harmful, since they presented higher callosity, which minimizes the rooting rates. It is indicated the addition of activated charcoal (1 g L-1) and lower concentration of agar (4 g L-1) in 50% MS culture medium for a better in vitro development of sequoia.Plantios experimentais com Sequoia sempervirens (Lamb), espécie conhecida mundialmente pelo grande porte, longevidade e qualidade madeireira, estão iniciando no Brasil, tornando necessário a disponibilidade de mudas de qualidade. Assim, a técnica de cultura de tecidos, via micropropagação, se torna uma grande aliada pela garantia de superioridade genética, produção em grande escala e tempo reduzido de propagação. Atualmente há inúmeros protocolos para diversas espécies, porém, é necessário verificar todos os agentes influentes no sucesso do desenvolvimento in vitro. O objetivo deste trabalho foi determinar a influência do carvão ativado e de concentrações de ágar no desenvolvimento in vitro aéreo e de raízes de S. sempervirens. Os tratamentos realizados envolveram a presença/ausência do carvão ativado (1 g L-1), combinados com concentrações de ágar (4, 5 e 6 g L-1) em meio MS 50%. Para o número e comprimento de brotos e de raízes, apenas o carvão ativado foi significativo, sendo que a presença beneficiou o desenvolvimento dos explantes. Para porcentagem de calo, houve interação dos fatores, sendo que maiores concentrações de ágar (5 e 6 g L-1) na presença do carvão foram prejudiciais, pois apresentaram maior calosidade, o que minimiza as taxas de enraizamento. Indica-se a adição de carvão ativado (1 g L-1) e de menor concentração de ágar (4 g L-1) em meio de cultura MS 50% para um melhor desenvolvimento in vitro de sequoia.Universidade do Estado de Santa Catarina2020-07-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.udesc.br/index.php/agroveterinaria/article/view/1079110.5965/2238117118e2019020Revista de Ciências Agroveterinárias; Vol. 18 (2019): Edição Especial - XII Simpósio Florestal Catarinense; 20 - 24Revista de Ciências Agroveterinárias; v. 18 (2019): Edição Especial - XII Simpósio Florestal Catarinense; 20 - 242238-1171reponame:Revista de Ciências Agroveterinárias (Online)instname:Universidade do Estado de Santa Catarina (UDESC)instacron:UDESCporhttps://periodicos.udesc.br/index.php/agroveterinaria/article/view/10791/pdfCopyright (c) 2019 Revista de Ciências Agroveterinárias (Journal of Agroveterinary Sciences)info:eu-repo/semantics/openAccessMeneguzzi, AlineCamargo, Samila SilvaNavroski, Marcio CarlosPereira, Mariane de OliveiraSouza, Patricia FukushimaRufato, Leo2022-04-29T13:56:20Zoai::article/10791Revistahttps://periodicos.udesc.br/index.php/agroveterinaria/PUBhttps://periodicos.udesc.br/index.php/agroveterinaria/oairca.cav@udesc.br \| portal.periodicos@udesc.brhttps://doi.org/10.5965/223811712238-11711676-9732opendoar:2022-04-29T13:56:20Revista de Ciências Agroveterinárias (Online) - Universidade do Estado de Santa Catarina (UDESC)false
score	15,198674

Agar and activated charcoal influence the in vitro development of shoot and root sequoia

Registos relacionados