Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): Preliminary characterization

Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L-methionine uroprophyrinogen I...

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Detalhes bibliográficos
Autores: Vannini, María Verónica, Rodríguez, Adriana, Vera, José Luis, de Valdéz, Graciela F., Taranto, Maria Pia, Sesma, Fernando Juan Manuel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2011
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/153705
Acesso em linha:http://hdl.handle.net/11336/153705
Access Level:Acceso aberto
Palavra-chave:COBALAMIN
LACTOBACILLUS REUTERI
UROPROPHYRINOGEN III METHYLTRANSFERASE
VITAMIN B 12
https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
Descrição
Resumo:Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L-methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.

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