Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi

Arginine kinase catalyzes the transphosphorylation between phosphoarginine and ADP. Phosphoarginine is involved in temporal ATP buffering and inorganic phosphate regulation. Trypanosoma cruzi arginine kinase phosphorylates only L‐arginine (specific activity 398.9 mUE‐min−1 mg−1), and is inhibited by...

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Detalhes bibliográficos
Autores: Pereira, Claudio Alejandro, Alonso, Guillermo Daniel, Ivaldi, María Soledad, Bouvier, León Alberto, Torres, Hector Norberto, Flawia, Mirtha Maria
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/43567
Acesso em linha:http://hdl.handle.net/11336/43567
Access Level:acceso abierto
Palavra-chave:Canavanine
Guanidino Kinase
Homoarginine
Phosphagen Kinase
Phosphoarginine
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_daa697e54e1eb73827ab7466e5c6d40d
oai_identifier_str oai:ri.conicet.gov.ar:11336/43567
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruziPereira, Claudio AlejandroAlonso, Guillermo DanielIvaldi, María SoledadBouvier, León AlbertoTorres, Hector NorbertoFlawia, Mirtha MariaCanavanineGuanidino KinaseHomoargininePhosphagen KinasePhosphoargininehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arginine kinase catalyzes the transphosphorylation between phosphoarginine and ADP. Phosphoarginine is involved in temporal ATP buffering and inorganic phosphate regulation. Trypanosoma cruzi arginine kinase phosphorylates only L‐arginine (specific activity 398.9 mUE‐min−1 mg−1), and is inhibited by the arginine analogs, agmatine, canavanine, nitroarginine, and homoarginine. Canavanine and homoarginine also produce a significant inhibition of the epimastigote culture growth (79.7% and 55.8%, respectively). Inhibition constants were calculated for canavanine and homoarginine (7.55 and 6.02 mM, respectively). In addition, two novel guanidino kinase activities were detected in the epimastigote soluble extract. The development of the arginine kinase inhibitors of T. cruzi could be an important feature because the phosphagens biosynthetic pathway in trypanosomatids is different from the one in their mammalian hosts.Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ivaldi, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Torres, Hector Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Flawia, Mirtha Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaWiley Blackwell Publishing, Inc2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43567Pereira, Claudio Alejandro; Alonso, Guillermo Daniel; Ivaldi, María Soledad; Bouvier, León Alberto; Torres, Hector Norberto; et al.; Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 50; 2; 12-2003; 132-1341066-5234CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1550-7408.2003.tb00247.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1550-7408.2003.tb00247.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:54:55Zoai:ri.conicet.gov.ar:11336/43567instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:54:55.371CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
title Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
spellingShingle Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
Pereira, Claudio Alejandro
Canavanine
Guanidino Kinase
Homoarginine
Phosphagen Kinase
Phosphoarginine
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
title_full Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
title_fullStr Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
title_full_unstemmed Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
title_sort Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi
dc.creator.none.fl_str_mv Pereira, Claudio Alejandro
Alonso, Guillermo Daniel
Ivaldi, María Soledad
Bouvier, León Alberto
Torres, Hector Norberto
Flawia, Mirtha Maria
author Pereira, Claudio Alejandro
author_facet Pereira, Claudio Alejandro
Alonso, Guillermo Daniel
Ivaldi, María Soledad
Bouvier, León Alberto
Torres, Hector Norberto
Flawia, Mirtha Maria
author_role author
author2 Alonso, Guillermo Daniel
Ivaldi, María Soledad
Bouvier, León Alberto
Torres, Hector Norberto
Flawia, Mirtha Maria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Canavanine
Guanidino Kinase
Homoarginine
Phosphagen Kinase
Phosphoarginine
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Canavanine
Guanidino Kinase
Homoarginine
Phosphagen Kinase
Phosphoarginine
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Arginine kinase catalyzes the transphosphorylation between phosphoarginine and ADP. Phosphoarginine is involved in temporal ATP buffering and inorganic phosphate regulation. Trypanosoma cruzi arginine kinase phosphorylates only L‐arginine (specific activity 398.9 mUE‐min−1 mg−1), and is inhibited by the arginine analogs, agmatine, canavanine, nitroarginine, and homoarginine. Canavanine and homoarginine also produce a significant inhibition of the epimastigote culture growth (79.7% and 55.8%, respectively). Inhibition constants were calculated for canavanine and homoarginine (7.55 and 6.02 mM, respectively). In addition, two novel guanidino kinase activities were detected in the epimastigote soluble extract. The development of the arginine kinase inhibitors of T. cruzi could be an important feature because the phosphagens biosynthetic pathway in trypanosomatids is different from the one in their mammalian hosts.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43567
Pereira, Claudio Alejandro; Alonso, Guillermo Daniel; Ivaldi, María Soledad; Bouvier, León Alberto; Torres, Hector Norberto; et al.; Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 50; 2; 12-2003; 132-134
1066-5234
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43567
identifier_str_mv Pereira, Claudio Alejandro; Alonso, Guillermo Daniel; Ivaldi, María Soledad; Bouvier, León Alberto; Torres, Hector Norberto; et al.; Screening of Substrate Analogs as Potential Enzyme Inhibitors for the Arginine Kinase of Trypanosoma cruzi; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 50; 2; 12-2003; 132-134
1066-5234
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1550-7408.2003.tb00247.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1550-7408.2003.tb00247.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,223283

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