Overcoming differences: The catalytic mechanism of metallo-β-lactamases

Metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. The worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. Inhibi...

Descripción completa

Detalles Bibliográficos
Autores: Meini, María Rocío, Llarrull, Leticia Irene, Vila, Alejandro Jose
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/52427
Acceso en línea:http://hdl.handle.net/11336/52427
Access Level:acceso abierto
Palabra clave:Antibiotic Resistance
Drug Design
Mechanism
Metallo-Β-Lactamase
Zinc Enzyme
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_b657f9fdcf1ebe9ca14936e77e09ed18
oai_identifier_str oai:ri.conicet.gov.ar:11336/52427
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Overcoming differences: The catalytic mechanism of metallo-β-lactamasesMeini, María RocíoLlarrull, Leticia IreneVila, Alejandro JoseAntibiotic ResistanceDrug DesignMechanismMetallo-Β-LactamaseZinc Enzymehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. The worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. Inhibitor design has been mostly impeded by the structural diversity of these enzymes. Here we provide a critical review of mechanistic studies of the three known subclasses of metallo-β-lactamases, analyzed at the light of structural and mutagenesis investigations. We propose that these enzymes present a modular structure in their active sites that can be dissected into two halves: one providing the attacking nucleophile, and the second one stabilizing a negatively charged reaction intermediate. These are common mechanistic elements in all metallo-β-lactamases. Nucleophile activation does not necessarily requires a Zn(II) ion, but a Zn(II) center is essential for stabilization of the anionic intermediate. Design of a common inhibitor could be therefore approached based in these convergent mechanistic features despite the structural differences.Fil: Meini, María Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Área Biofísica; ArgentinaFil: Llarrull, Leticia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Área Biofísica; ArgentinaFil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Área Biofísica; ArgentinaElsevier Science2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52427Meini, María Rocío; Llarrull, Leticia Irene; Vila, Alejandro Jose; Overcoming differences: The catalytic mechanism of metallo-β-lactamases; Elsevier Science; FEBS Letters; 589; 22; 7-2015; 3419-34320014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2015.08.015info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2015.08.015info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640939/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:40:20Zoai:ri.conicet.gov.ar:11336/52427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:40:21.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Overcoming differences: The catalytic mechanism of metallo-β-lactamases
title Overcoming differences: The catalytic mechanism of metallo-β-lactamases
spellingShingle Overcoming differences: The catalytic mechanism of metallo-β-lactamases
Meini, María Rocío
Antibiotic Resistance
Drug Design
Mechanism
Metallo-Β-Lactamase
Zinc Enzyme
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Overcoming differences: The catalytic mechanism of metallo-β-lactamases
title_full Overcoming differences: The catalytic mechanism of metallo-β-lactamases
title_fullStr Overcoming differences: The catalytic mechanism of metallo-β-lactamases
title_full_unstemmed Overcoming differences: The catalytic mechanism of metallo-β-lactamases
title_sort Overcoming differences: The catalytic mechanism of metallo-β-lactamases
dc.creator.none.fl_str_mv Meini, María Rocío
Llarrull, Leticia Irene
Vila, Alejandro Jose
author Meini, María Rocío
author_facet Meini, María Rocío
Llarrull, Leticia Irene
Vila, Alejandro Jose
author_role author
author2 Llarrull, Leticia Irene
Vila, Alejandro Jose
author2_role author
author
dc.subject.none.fl_str_mv Antibiotic Resistance
Drug Design
Mechanism
Metallo-Β-Lactamase
Zinc Enzyme
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Antibiotic Resistance
Drug Design
Mechanism
Metallo-Β-Lactamase
Zinc Enzyme
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. The worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. Inhibitor design has been mostly impeded by the structural diversity of these enzymes. Here we provide a critical review of mechanistic studies of the three known subclasses of metallo-β-lactamases, analyzed at the light of structural and mutagenesis investigations. We propose that these enzymes present a modular structure in their active sites that can be dissected into two halves: one providing the attacking nucleophile, and the second one stabilizing a negatively charged reaction intermediate. These are common mechanistic elements in all metallo-β-lactamases. Nucleophile activation does not necessarily requires a Zn(II) ion, but a Zn(II) center is essential for stabilization of the anionic intermediate. Design of a common inhibitor could be therefore approached based in these convergent mechanistic features despite the structural differences.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52427
Meini, María Rocío; Llarrull, Leticia Irene; Vila, Alejandro Jose; Overcoming differences: The catalytic mechanism of metallo-β-lactamases; Elsevier Science; FEBS Letters; 589; 22; 7-2015; 3419-3432
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52427
identifier_str_mv Meini, María Rocío; Llarrull, Leticia Irene; Vila, Alejandro Jose; Overcoming differences: The catalytic mechanism of metallo-β-lactamases; Elsevier Science; FEBS Letters; 589; 22; 7-2015; 3419-3432
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2015.08.015
info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2015.08.015
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640939/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194972036005888
score 15,812455

Similares en LA Referencia