Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes

Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P4...

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Detalhes bibliográficos
Autores: Huarte Bonnet, Carla, Kumar, Suresh, Saparrat, Mario Carlos Nazareno, Girotti, Juan Roberto, Santana, Marianela, Hallsworth, John E., Pedrini, Nicolás
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Recursos:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/141006
Acesso em linha:http://sedici.unlp.edu.ar/handle/10915/141006
Access Level:acceso abierto
Palavra-chave:Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
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spelling Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 GenesHuarte Bonnet, CarlaKumar, SureshSaparrat, Mario Carlos NazarenoGirotti, Juan RobertoSantana, MarianelaHallsworth, John E.Pedrini, NicolásBiologíaCiencias NaturalesAspergillus nigerBeauveria bassianaEntomopathogenic fungiHydrocarbon degradationMetarhizium anisopliaePenicillium chrysogenumSeveral filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Fisiología VegetalFacultad de Ciencias Agrarias y ForestalesInstituto de Botánica "Dr. Carlos Spegazzini"2017-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1047-1060http://sedici.unlp.edu.ar/handle/10915/141006enginfo:eu-repo/semantics/altIdentifier/issn/1559-0291info:eu-repo/semantics/altIdentifier/issn/0273-2289info:eu-repo/semantics/altIdentifier/doi/10.1007/s12010-017-2608-zinfo:eu-repo/semantics/altIdentifier/pmid/28942502info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:10:39Zoai:sedici.unlp.edu.ar:10915/141006Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:10:39.226SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
spellingShingle Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
Huarte Bonnet, Carla
Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
title_short Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_full Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_fullStr Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_full_unstemmed Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_sort Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
dc.creator.none.fl_str_mv Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author Huarte Bonnet, Carla
author_facet Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author_role author
author2 Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
topic Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
description Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141006
url http://sedici.unlp.edu.ar/handle/10915/141006
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1559-0291
info:eu-repo/semantics/altIdentifier/issn/0273-2289
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12010-017-2608-z
info:eu-repo/semantics/altIdentifier/pmid/28942502
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1047-1060
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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