Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2023 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/227389 |
| Online Access: | http://hdl.handle.net/11336/227389 |
| Access Level: | Open access |
| Keyword: | ANCESTRAL SEQUENCE RECONSTRUCTION ENZYME BIOTECHNOLOGY ENZYME EVOLUTION MECHANISTIC ENZYMOLOGY PROTEIN STABILITY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Summary: | Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide. |
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