The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies

We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor-Like Protein1 (VSR-1/ATELP1) receptor during the formation of protein storage vacuoles in <i>Arabidopsis thaliana</i> embryos by means...

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Authors: Otegui, Marisa, Herder, Rachel, Schulze, Jan, Jung, Rudolf, Staehelin, L. Andrew
Format: article
Status:Published version
Publication Date:2006
Country:Argentina
Institution:Universidad Nacional de La Plata
Repository:SEDICI (UNLP)
Language:English
OAI Identifier:oai:sedici.unlp.edu.ar:10915/83003
Online Access:http://sedici.unlp.edu.ar/handle/10915/83003
Access Level:Open access
Keyword:Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
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spelling The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodiesOtegui, MarisaHerder, RachelSchulze, JanJung, RudolfStaehelin, L. AndrewCiencias Naturalesstorage proteinsArabidopsis thaliana embryosWe have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor-Like Protein1 (VSR-1/ATELP1) receptor during the formation of protein storage vacuoles in <i>Arabidopsis thaliana</i> embryos by means of high-pressure freezing/freeze substitution, electron tomography, immunolabeling techniques, and subcellular fractionation. The storage proteins and their processing proteases are segregated from each other within the Golgi cisternae and packaged into separate vesicles. The storage protein-containing vesicles but not the processing enzyme-containing vesicles carry the VSR-1/ATELP1 receptor. Both types of secretory vesicles appear to fuse into a type of prevacuolar multivesicular body (MVB). We have also determined that the proteolytic processing of the 2S albumins starts in the MVBs. We hypothesize that the compartmentalized processing of storage proteins in the MVBs may allow for the sequential activation of processing proteases as the MVB lumen gradually acidifies.Instituto de Fisiología VegetalFacultad de Ciencias Naturales y Museo2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2567-2581http://sedici.unlp.edu.ar/handle/10915/83003enginfo:eu-repo/semantics/altIdentifier/issn/1040-4651info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.106.040931info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:53:49Zoai:sedici.unlp.edu.ar:10915/83003Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:53:49.499SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
spellingShingle The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
Otegui, Marisa
Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
title_short The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_full The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_fullStr The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_full_unstemmed The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_sort The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
dc.creator.none.fl_str_mv Otegui, Marisa
Herder, Rachel
Schulze, Jan
Jung, Rudolf
Staehelin, L. Andrew
author Otegui, Marisa
author_facet Otegui, Marisa
Herder, Rachel
Schulze, Jan
Jung, Rudolf
Staehelin, L. Andrew
author_role author
author2 Herder, Rachel
Schulze, Jan
Jung, Rudolf
Staehelin, L. Andrew
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
topic Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
description We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor-Like Protein1 (VSR-1/ATELP1) receptor during the formation of protein storage vacuoles in <i>Arabidopsis thaliana</i> embryos by means of high-pressure freezing/freeze substitution, electron tomography, immunolabeling techniques, and subcellular fractionation. The storage proteins and their processing proteases are segregated from each other within the Golgi cisternae and packaged into separate vesicles. The storage protein-containing vesicles but not the processing enzyme-containing vesicles carry the VSR-1/ATELP1 receptor. Both types of secretory vesicles appear to fuse into a type of prevacuolar multivesicular body (MVB). We have also determined that the proteolytic processing of the 2S albumins starts in the MVBs. We hypothesize that the compartmentalized processing of storage proteins in the MVBs may allow for the sequential activation of processing proteases as the MVB lumen gradually acidifies.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83003
url http://sedici.unlp.edu.ar/handle/10915/83003
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1040-4651
info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.106.040931
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
2567-2581
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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