Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates

The influence of culture medium composition on chromium(VI) quantification according to diphenylcarbazide (DPC) colorimetric determination was evaluated. Considering the eventual biospeciation of Cr(VI) as a mechanism of microbial bioremediation, the possibility to quantify Cr(III) in culture medium...

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Detalles Bibliográficos
Autores: Fernandez, Pablo Marcelo, Castellanos, Lucia Ines, Fariña, Julia Ines
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/41439
Acceso en línea:http://hdl.handle.net/11336/41439
Access Level:acceso abierto
Palabra clave:Cr(Vi)
Yeast
Fungi
Culture Medium
Cr(Iii) Oxidation
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal IsolatesFernandez, Pablo MarceloCastellanos, Lucia InesFariña, Julia InesCr(Vi)YeastFungiCulture MediumCr(Iii) Oxidationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The influence of culture medium composition on chromium(VI) quantification according to diphenylcarbazide (DPC) colorimetric determination was evaluated. Considering the eventual biospeciation of Cr(VI) as a mechanism of microbial bioremediation, the possibility to quantify Cr(III) in culture medium was also explored. Yeast nitrogen base (YNB) was identified as the least interferent culture medium for Cr(VI) quantification by DPC and it was applied to compare different strategies for Cr(III) oxidation. The most appropriate oxidation protocol consisted in the reaction with 80 mM KIO4 at room temperature for 30 min prior to DPC. Parameters like basal culture medium (vitamins + salts + oligoelements), C and N source were systematically evaluated, either independently or in combination. Results demonstrated that C source was the most interferent culture medium component, being the use of sucrose preferable to glucose. A medium arbitrarily named as YNB′ (YNB without amino acids and ammonium sulfate plus 50 g L−1 sucrose and 0.6 g L−1 (NH4)2SO4) was defined for Cr(VI)-amended fungal cultures. Kinetics of growth, Cr(VI) removal, and nutrient consumption for isolates A. pullulans VR-8, filamentous fungus PMF-1, and Lecythophora sp. NGV-1 were obtained. The order of Cr(VI) removal efficiency was as follows: A. pullulans VR-8 > Lecythophora sp. NGV-1 > filamentous fungus PMF-1, and a similar trend was observed for biomass yield and nutrients consumption. Studies on biospeciation by means of the selected Cr(III) oxidation protocol were unsuccessful, leading to Cr(VI) values much lower than expected. It revealed that this kind of protocols should be cautiously evaluated when studying microbial Cr(VI) bioremediation.Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaSpringer2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41439Fernandez, Pablo Marcelo; Castellanos, Lucia Ines; Fariña, Julia Ines; Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates; Springer; Water, Air and Soil Pollution; 206; 1-4; 2-2010; 283-2930049-6979CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-009-0105-xinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11270-009-0105-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:42:38Zoai:ri.conicet.gov.ar:11336/41439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:42:38.227CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
title Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
spellingShingle Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
Fernandez, Pablo Marcelo
Cr(Vi)
Yeast
Fungi
Culture Medium
Cr(Iii) Oxidation
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
title_full Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
title_fullStr Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
title_full_unstemmed Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
title_sort Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates
dc.creator.none.fl_str_mv Fernandez, Pablo Marcelo
Castellanos, Lucia Ines
Fariña, Julia Ines
author Fernandez, Pablo Marcelo
author_facet Fernandez, Pablo Marcelo
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
dc.subject.none.fl_str_mv Cr(Vi)
Yeast
Fungi
Culture Medium
Cr(Iii) Oxidation
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Cr(Vi)
Yeast
Fungi
Culture Medium
Cr(Iii) Oxidation
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The influence of culture medium composition on chromium(VI) quantification according to diphenylcarbazide (DPC) colorimetric determination was evaluated. Considering the eventual biospeciation of Cr(VI) as a mechanism of microbial bioremediation, the possibility to quantify Cr(III) in culture medium was also explored. Yeast nitrogen base (YNB) was identified as the least interferent culture medium for Cr(VI) quantification by DPC and it was applied to compare different strategies for Cr(III) oxidation. The most appropriate oxidation protocol consisted in the reaction with 80 mM KIO4 at room temperature for 30 min prior to DPC. Parameters like basal culture medium (vitamins + salts + oligoelements), C and N source were systematically evaluated, either independently or in combination. Results demonstrated that C source was the most interferent culture medium component, being the use of sucrose preferable to glucose. A medium arbitrarily named as YNB′ (YNB without amino acids and ammonium sulfate plus 50 g L−1 sucrose and 0.6 g L−1 (NH4)2SO4) was defined for Cr(VI)-amended fungal cultures. Kinetics of growth, Cr(VI) removal, and nutrient consumption for isolates A. pullulans VR-8, filamentous fungus PMF-1, and Lecythophora sp. NGV-1 were obtained. The order of Cr(VI) removal efficiency was as follows: A. pullulans VR-8 > Lecythophora sp. NGV-1 > filamentous fungus PMF-1, and a similar trend was observed for biomass yield and nutrients consumption. Studies on biospeciation by means of the selected Cr(III) oxidation protocol were unsuccessful, leading to Cr(VI) values much lower than expected. It revealed that this kind of protocols should be cautiously evaluated when studying microbial Cr(VI) bioremediation.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41439
Fernandez, Pablo Marcelo; Castellanos, Lucia Ines; Fariña, Julia Ines; Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates; Springer; Water, Air and Soil Pollution; 206; 1-4; 2-2010; 283-293
0049-6979
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41439
identifier_str_mv Fernandez, Pablo Marcelo; Castellanos, Lucia Ines; Fariña, Julia Ines; Critical Influence of Culture Medium and Cr(III) Quantification Protocols on the Interpretation of Cr(VI) Bioremediation by Environmental Fungal Isolates; Springer; Water, Air and Soil Pollution; 206; 1-4; 2-2010; 283-293
0049-6979
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-009-0105-x
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11270-009-0105-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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