The flagellar adenylate kinases of Trypanosoma cruzi

Adenylate kinases (ADK) are key enzymes involved in cell energy management. Trypanosomatids present the highest number of variants in a single cell in comparison with the rest of the living organisms. In this work, we characterized two flagellar ADKs from Trypanosoma cruzi, called TcADK1 and TcADK4,...

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Detalhes bibliográficos
Autores: Camara, María de los Milagros, Bouvier, León Alberto, Miranda, Mariana Reneé, Pereira, Claudio Alejandro
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/20357
Acesso em linha:http://hdl.handle.net/11336/20357
Access Level:acceso abierto
Palavra-chave:Chagas Disease
Trypanosoma Brucei
Trypanosoma Cruzi
Adenylate Kinase
Flagellum
Phosphotransferase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20357
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling The flagellar adenylate kinases of Trypanosoma cruziCamara, María de los MilagrosBouvier, León AlbertoMiranda, Mariana ReneéPereira, Claudio AlejandroChagas DiseaseTrypanosoma BruceiTrypanosoma CruziAdenylate KinaseFlagellumPhosphotransferasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adenylate kinases (ADK) are key enzymes involved in cell energy management. Trypanosomatids present the highest number of variants in a single cell in comparison with the rest of the living organisms. In this work, we characterized two flagellar ADKs from Trypanosoma cruzi, called TcADK1 and TcADK4, which are also located in the cell cytosol. Interestingly, TcADK1 presents a stage-specific expression. This variant was detected in epimastigotes cells, and was completely absent in trypomastigotes and amastigotes, while TcADK4 is present in the major life cycle stages of T. cruzi. Both variants are also regulated, in opposite ways, along the parasite growth curve suggesting that their expression depends on the intra- and extracellular conditions. Both, TcADK1 and TcADK4 present N-terminal extension that could be responsible for their subcellular localization. The presence of ADK variants in the flagellum would be critical for the provision of energy in a process of high ATP consumption such as cell motility.Fil: Camara, María de los Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaOxford University Press2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20357Camara, María de los Milagros; Bouvier, León Alberto; Miranda, Mariana Reneé; Pereira, Claudio Alejandro; The flagellar adenylate kinases of Trypanosoma cruzi; Oxford University Press; FEMS Microbiology Letters; 362; 1; 1-2015; 1-50378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/362/1/1/2467431/The-manganese-responsive-regulator-MntR-repressesinfo:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnu020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:34:55Zoai:ri.conicet.gov.ar:11336/20357instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:34:55.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The flagellar adenylate kinases of Trypanosoma cruzi
title The flagellar adenylate kinases of Trypanosoma cruzi
spellingShingle The flagellar adenylate kinases of Trypanosoma cruzi
Camara, María de los Milagros
Chagas Disease
Trypanosoma Brucei
Trypanosoma Cruzi
Adenylate Kinase
Flagellum
Phosphotransferase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short The flagellar adenylate kinases of Trypanosoma cruzi
title_full The flagellar adenylate kinases of Trypanosoma cruzi
title_fullStr The flagellar adenylate kinases of Trypanosoma cruzi
title_full_unstemmed The flagellar adenylate kinases of Trypanosoma cruzi
title_sort The flagellar adenylate kinases of Trypanosoma cruzi
dc.creator.none.fl_str_mv Camara, María de los Milagros
Bouvier, León Alberto
Miranda, Mariana Reneé
Pereira, Claudio Alejandro
author Camara, María de los Milagros
author_facet Camara, María de los Milagros
Bouvier, León Alberto
Miranda, Mariana Reneé
Pereira, Claudio Alejandro
author_role author
author2 Bouvier, León Alberto
Miranda, Mariana Reneé
Pereira, Claudio Alejandro
author2_role author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Trypanosoma Brucei
Trypanosoma Cruzi
Adenylate Kinase
Flagellum
Phosphotransferase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Chagas Disease
Trypanosoma Brucei
Trypanosoma Cruzi
Adenylate Kinase
Flagellum
Phosphotransferase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Adenylate kinases (ADK) are key enzymes involved in cell energy management. Trypanosomatids present the highest number of variants in a single cell in comparison with the rest of the living organisms. In this work, we characterized two flagellar ADKs from Trypanosoma cruzi, called TcADK1 and TcADK4, which are also located in the cell cytosol. Interestingly, TcADK1 presents a stage-specific expression. This variant was detected in epimastigotes cells, and was completely absent in trypomastigotes and amastigotes, while TcADK4 is present in the major life cycle stages of T. cruzi. Both variants are also regulated, in opposite ways, along the parasite growth curve suggesting that their expression depends on the intra- and extracellular conditions. Both, TcADK1 and TcADK4 present N-terminal extension that could be responsible for their subcellular localization. The presence of ADK variants in the flagellum would be critical for the provision of energy in a process of high ATP consumption such as cell motility.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20357
Camara, María de los Milagros; Bouvier, León Alberto; Miranda, Mariana Reneé; Pereira, Claudio Alejandro; The flagellar adenylate kinases of Trypanosoma cruzi; Oxford University Press; FEMS Microbiology Letters; 362; 1; 1-2015; 1-5
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20357
identifier_str_mv Camara, María de los Milagros; Bouvier, León Alberto; Miranda, Mariana Reneé; Pereira, Claudio Alejandro; The flagellar adenylate kinases of Trypanosoma cruzi; Oxford University Press; FEMS Microbiology Letters; 362; 1; 1-2015; 1-5
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/362/1/1/2467431/The-manganese-responsive-regulator-MntR-represses
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnu020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.223283

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