Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

In this work, the localization, density, morphology and ultrastructure of secretory structures in aerial organs of Flourensia campestris (FC) and F. oolepis (FO) (Asteraceae) by means of a combination of light, fluorescence, transmission (TEM) and scanning electron microscopy (SEM) were examined. Th...

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Detalhes bibliográficos
Autores: Silva, Mariana Paola, Tourn, Graciela Monica, López, Daniela Griselda, Galati, Beatriz Gloria, Piazza, Leonardo A, Zarlavsky, Gabriela Elena, Cantero, Juan Jose, Scopel, Ana Leonor
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2015
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/46852
Acesso em linha:http://hdl.handle.net/11336/46852
Access Level:Acceso aberto
Palavra-chave:Flourensia campestris
Flourensia oolepis
Glandular Trichomes
Secretory Ducts
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46852
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
title Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
spellingShingle Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
Silva, Mariana Paola
Flourensia campestris
Flourensia oolepis
Glandular Trichomes
Secretory Ducts
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
title_full Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
title_fullStr Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
title_full_unstemmed Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
title_sort Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion
dc.creator.none.fl_str_mv Silva, Mariana Paola
Tourn, Graciela Monica
López, Daniela Griselda
Galati, Beatriz Gloria
Piazza, Leonardo A
Zarlavsky, Gabriela Elena
Cantero, Juan Jose
Scopel, Ana Leonor
author Silva, Mariana Paola
author_facet Silva, Mariana Paola
Tourn, Graciela Monica
López, Daniela Griselda
Galati, Beatriz Gloria
Piazza, Leonardo A
Zarlavsky, Gabriela Elena
Cantero, Juan Jose
Scopel, Ana Leonor
author_role author
author2 Tourn, Graciela Monica
López, Daniela Griselda
Galati, Beatriz Gloria
Piazza, Leonardo A
Zarlavsky, Gabriela Elena
Cantero, Juan Jose
Scopel, Ana Leonor
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Flourensia campestris
Flourensia oolepis
Glandular Trichomes
Secretory Ducts
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Flourensia campestris
Flourensia oolepis
Glandular Trichomes
Secretory Ducts
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description In this work, the localization, density, morphology and ultrastructure of secretory structures in aerial organs of Flourensia campestris (FC) and F. oolepis (FO) (Asteraceae) by means of a combination of light, fluorescence, transmission (TEM) and scanning electron microscopy (SEM) were examined. The possible role of secretory structures in the production and secretion of the phytotoxic sesquiterpene (-)-hamanasic acid A ((-)HAA) in both species was also assessed. Capitate glandular trichomes were found in all reproductive organs of FC and FO, and were being reported for the first time. These glandular trichomes, typically associated to edges and veins, were of the same type as those already described for vegetative organs, and were abundant in involucral bracts and corolla of tubulose and ligulate flowers. Their density in reproductive organs of both species was similar (ca. 30/mm2) and lower than that found in leaves (ca. 100/mm2) and stems (ca. 160/mm2 in FC, and up to 650/mm2 in FO). Glandular trichomes in vegetative organs followed a species- specific pattern of distribution. TEM and SEM observations suggest that each species differs in the way in which secretory materials are released to the outside: through cracks or pores in FC, or through a loose cuticle in FO. Similar inspections of the secretory ducts revealed lipophilic vacuoles localized in subepithelial and epithelial cells, in which secretions accumulated before being transferred to the duct. The presence of wall ingrowths in subepithelial cells suggests that granulocrine secretion operates in these species. Secretory ducts varied in density and diameter among the organs in both species, with the combination being maximal in woody stems. (-)HAA was only detected in surface secreted resins of both species, and its concentration (2D-TLC, GC-FID) was intimately associated with the distribution and density of glandular trichomes in each organ (capitula, leaves, and stems with primary or secondary growth). In addition, no (-)HAA was detected internally in the resins collected from secretory ducts. The composition of these resins showed distinctive profiles for FC and FO, and only four from ca. 30 compounds detected (GC/MS) were shared by both species. In addition to the elucidation of ultrastructural traits, distribution and density of secretory structures in aerial organs of FC and FO, present findings suggest a functional role for glandular trichomes in the secretion of the putative phytotoxic allelochemical (-)HAA.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46852
Silva, Mariana Paola; Tourn, Graciela Monica; López, Daniela Griselda; Galati, Beatriz Gloria; Piazza, Leonardo A; et al.; Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion; Scientific Research Publishing; American Journal of Plant Sciences; 6; 7; 14-4-2015; 925-942
2158-2742
2158-2750
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46852
identifier_str_mv Silva, Mariana Paola; Tourn, Graciela Monica; López, Daniela Griselda; Galati, Beatriz Gloria; Piazza, Leonardo A; et al.; Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion; Scientific Research Publishing; American Journal of Plant Sciences; 6; 7; 14-4-2015; 925-942
2158-2742
2158-2750
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2015.67100
info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/11-2602013_55600.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196138419519488
spelling Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A SecretionSilva, Mariana PaolaTourn, Graciela MonicaLópez, Daniela GriseldaGalati, Beatriz GloriaPiazza, Leonardo AZarlavsky, Gabriela ElenaCantero, Juan JoseScopel, Ana LeonorFlourensia campestrisFlourensia oolepisGlandular TrichomesSecretory Ductshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work, the localization, density, morphology and ultrastructure of secretory structures in aerial organs of Flourensia campestris (FC) and F. oolepis (FO) (Asteraceae) by means of a combination of light, fluorescence, transmission (TEM) and scanning electron microscopy (SEM) were examined. The possible role of secretory structures in the production and secretion of the phytotoxic sesquiterpene (-)-hamanasic acid A ((-)HAA) in both species was also assessed. Capitate glandular trichomes were found in all reproductive organs of FC and FO, and were being reported for the first time. These glandular trichomes, typically associated to edges and veins, were of the same type as those already described for vegetative organs, and were abundant in involucral bracts and corolla of tubulose and ligulate flowers. Their density in reproductive organs of both species was similar (ca. 30/mm2) and lower than that found in leaves (ca. 100/mm2) and stems (ca. 160/mm2 in FC, and up to 650/mm2 in FO). Glandular trichomes in vegetative organs followed a species- specific pattern of distribution. TEM and SEM observations suggest that each species differs in the way in which secretory materials are released to the outside: through cracks or pores in FC, or through a loose cuticle in FO. Similar inspections of the secretory ducts revealed lipophilic vacuoles localized in subepithelial and epithelial cells, in which secretions accumulated before being transferred to the duct. The presence of wall ingrowths in subepithelial cells suggests that granulocrine secretion operates in these species. Secretory ducts varied in density and diameter among the organs in both species, with the combination being maximal in woody stems. (-)HAA was only detected in surface secreted resins of both species, and its concentration (2D-TLC, GC-FID) was intimately associated with the distribution and density of glandular trichomes in each organ (capitula, leaves, and stems with primary or secondary growth). In addition, no (-)HAA was detected internally in the resins collected from secretory ducts. The composition of these resins showed distinctive profiles for FC and FO, and only four from ca. 30 compounds detected (GC/MS) were shared by both species. In addition to the elucidation of ultrastructural traits, distribution and density of secretory structures in aerial organs of FC and FO, present findings suggest a functional role for glandular trichomes in the secretion of the putative phytotoxic allelochemical (-)HAA.Fil: Silva, Mariana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Sede Polo Universitario Punilla Centro; ArgentinaFil: Tourn, Graciela Monica. Universidad de Buenos Aires. Facultad de Agronomia. Sede Polo Universitario Punilla Centro; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Botánica Agrícola; ArgentinaFil: López, Daniela Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Sede Polo Universitario Punilla Centro; ArgentinaFil: Galati, Beatriz Gloria. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Botánica Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Piazza, Leonardo A. Universidad de Buenos Aires. Facultad de Agronomia. Sede Polo Universitario Punilla Centro; ArgentinaFil: Zarlavsky, Gabriela Elena. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Botánica Agrícola; ArgentinaFil: Cantero, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Biología Agrícola; ArgentinaFil: Scopel, Ana Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Sede Polo Universitario Punilla Centro; ArgentinaScientific Research Publishing2015-04-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46852Silva, Mariana Paola; Tourn, Graciela Monica; López, Daniela Griselda; Galati, Beatriz Gloria; Piazza, Leonardo A; et al.; Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion; Scientific Research Publishing; American Journal of Plant Sciences; 6; 7; 14-4-2015; 925-9422158-27422158-2750CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2015.67100info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/11-2602013_55600.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:16:06Zoai:ri.conicet.gov.ar:11336/46852instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:16:06.993CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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