Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data

In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer...

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Detalles Bibliográficos
Autores: Gottlieb, A.M., Giberti, G.C., Poggio, L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Argentina
Institución:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
Repositorio:Biblioteca Digital (UBA-FCEN)
Idioma:inglés
OAI Identifier:paperaa:paper_00029122_v92_n2_p352_Gottlieb
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb
Access Level:acceso abierto
Palabra clave:AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex argentina
Ilex brasiliensis
Ilex brevicuspis
Ilex dumosa
Ilex integerrima
Ilex paraguariensis
Ilex theezans
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oai_identifier_str paperaa:paper_00029122_v92_n2_p352_Gottlieb
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spelling Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence dataGottlieb, A.M.Giberti, G.C.Poggio, L.AFLPDirect optimizationIlexITSSensitivity analysismolecular analysissensitivity analysistaxonomyAquifoliaceaeEmbryophytaIlexIlexIlex argentinaIlex argentinaIlex brasiliensisIlex brasiliensisIlex brevicuspisIlex brevicuspisIlex dumosaIlex dumosaIlex integerrimaIlex integerrimaIlex paraguariensisIlex paraguariensisIlex theezansIlex theezansIn order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found.Fil:Gottlieb, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_GottliebAm. J. Bot. 2005;92(2):352-369reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2024-05-10T10:42:41Zpaperaa:paper_00029122_v92_n2_p352_GottliebInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962024-05-10 10:42:43.031Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
spellingShingle Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
Gottlieb, A.M.
AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans
title_short Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_full Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_fullStr Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_full_unstemmed Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_sort Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
dc.creator.none.fl_str_mv Gottlieb, A.M.
Giberti, G.C.
Poggio, L.
author Gottlieb, A.M.
author_facet Gottlieb, A.M.
Giberti, G.C.
Poggio, L.
author_role author
author2 Giberti, G.C.
Poggio, L.
author2_role author
author
dc.subject.none.fl_str_mv AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans
topic AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans
description In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb
url http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Am. J. Bot. 2005;92(2):352-369
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 15.223283

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