Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
A new methodology for caffeine determination was developed based on the quenching effect on fluorescent emission of the molecule of Bovine Serum Albumin at λem = 338 nm (λex = 280). A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium di...
| Authors: | , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2016 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/60470 |
| Online Access: | http://hdl.handle.net/11336/60470 |
| Access Level: | Open access |
| Keyword: | Bovine Serum Albumin Caffeine Determination Energy Supplements Flow Injection Analysis Molecular Fluorescence https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Summary: | A new methodology for caffeine determination was developed based on the quenching effect on fluorescent emission of the molecule of Bovine Serum Albumin at λem = 338 nm (λex = 280). A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium dihydrophosphate 5 · 10-3 mol L-1 buffer (pH 6.8) as carrier and flow rate of 1.5 mL min-1. The experimental and instrumental conditions that influence on analytical quality parameters were systematically investigated, as consider: buffer nature and concentration, fluorophore nature and concentration, and carrier flow rate. The proposal is simple, fast, inexpensive and precise, with a linear range from 6.68 · 10-6 to 4.0 · 10-3 mol L-1 and SD of 0.0668, under optimized conditions. Methodology sensibility and selectivity allowed a variety of sample analyses. It was successfully applied to caffeine quantification in energy drinks, dietary supplements and sliming infusion samples without previous treatment. |
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