Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms

This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or ca...

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Bibliographic Details
Authors: Blanco Prieto, Olga, Catalán, Jaime, Trujillo Rojas, Lina, Peña García, Àlex, Rivera del Álamo, Ma. Montserrat, Llavanera, Marc, Bonet, Sergi, Fernández Novell, Josep Maria, Yeste Oliveras, Marc, Rodríguez Gil, Joan Enric
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/18733
Online Access:http://hdl.handle.net/10256/18733
Access Level:Open access
Keyword:Espermatozoides
Mitocondris
Spermatozoa
Mitochondria
Citocrom c
Cytochrome c
Porcs -- Espermatozoides
Swine -- Spermatozoa
Description
Summary:This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner

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