Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)

Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad

Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)

We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximu...

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Detalhes bibliográficos
Autores:	Lopez Mañanes, Alejandra Antonia, del Valle, Juana Cristina, Michiels, Maria Soledad
Formato:	artículo
Estado:	Versión publicada
Fecha de publicación:	2015
País:	Argentina
Recursos:	Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:	CONICET Digital (CONICET)
Idioma:	inglés
OAI Identifier:	oai:ri.conicet.gov.ar:11336/39856
Acesso em linha:	http://hdl.handle.net/11336/39856
Access Level:	acceso abierto
Palavra-chave:	Intertidal Euryhaline Crabs Neohelice Granulata Dopamine Glucagon Camp Hepatopancreas Enzymes https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1

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network_name_str	Argentina
repository_id_str
spelling	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)Lopez Mañanes, Alejandra Antoniadel Valle, Juana CristinaMichiels, Maria SoledadIntertidal Euryhaline CrabsNeohelice GranulataDopamineGlucagonCampHepatopancreasEnzymeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: del Valle, Juana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Michiels, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaBrill Academic Publishers2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39856Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad; Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae); Brill Academic Publishers; Crustaceana; 88; 1; 1-2015; 51-650011-216XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1163/15685403-00003399info:eu-repo/semantics/altIdentifier/url/http://booksandjournals.brillonline.com/content/journals/10.1163/15685403-00003399info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:43:04Zoai:ri.conicet.gov.ar:11336/39856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:43:05.154CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
spellingShingle	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae) Lopez Mañanes, Alejandra Antonia Intertidal Euryhaline Crabs Neohelice Granulata Dopamine Glucagon Camp Hepatopancreas Enzymes https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
title_short	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_full	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_fullStr	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_full_unstemmed	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_sort	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
dc.creator.none.fl_str_mv	Lopez Mañanes, Alejandra Antonia del Valle, Juana Cristina Michiels, Maria Soledad
author	Lopez Mañanes, Alejandra Antonia
author_facet	Lopez Mañanes, Alejandra Antonia del Valle, Juana Cristina Michiels, Maria Soledad
author_role	author
author2	del Valle, Juana Cristina Michiels, Maria Soledad
author2_role	author author
dc.subject.none.fl_str_mv	Intertidal Euryhaline Crabs Neohelice Granulata Dopamine Glucagon Camp Hepatopancreas Enzymes https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
topic	Intertidal Euryhaline Crabs Neohelice Granulata Dopamine Glucagon Camp Hepatopancreas Enzymes https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
description	We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.
publishDate	2015
dc.date.none.fl_str_mv	2015-01
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/39856 Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad; Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae); Brill Academic Publishers; Crustaceana; 88; 1; 1-2015; 51-65 0011-216X CONICET Digital CONICET
url	http://hdl.handle.net/11336/39856
identifier_str_mv	Lopez Mañanes, Alejandra Antonia; del Valle, Juana Cristina; Michiels, Maria Soledad; Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae); Brill Academic Publishers; Crustaceana; 88; 1; 1-2015; 51-65 0011-216X CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1163/15685403-00003399 info:eu-repo/semantics/altIdentifier/url/http://booksandjournals.brillonline.com/content/journals/10.1163/15685403-00003399
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Brill Academic Publishers
publisher.none.fl_str_mv	Brill Academic Publishers
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	15,812429

Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)

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